FastQCFastQC Report
Thu 20 Feb 2025
POR-260-TP2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenamePOR-260-TP2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17539918
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAA1805981.0296399333223794No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1702830.9708312205336421No Hit
CAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAAGGTTCCATG919160.5240389379243392No Hit
GTCTAACATGTGCGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATG904480.5156694575196988No Hit
CGCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACG711620.4057145535115957No Hit
CTTGGATTTACGAAAGACGAACTACTGCGAAAGCATTTGCCAAGAATGTT685320.39072018466676983No Hit
GACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGT642650.3663928189402026No Hit
GCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATT600430.34232201085546693No Hit
CTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGG521670.2974187222540037No Hit
TGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAATGG518770.2957653507844221No Hit
AGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAA504390.28756690880766944No Hit
CAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTC500430.2853092015595512No Hit
TGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC487220.2777778094515607No Hit
CGCAGATTCAGACAATTGGCATTTGCACTTGCCTGAAAAGACAATGGTGC475730.27122703766346No Hit
CTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAACTAACAAGGATTC426730.2432907611084613No Hit
CAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAGCTCAAATTTGA380250.21679120734771962No Hit
GTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAG375640.21416291683917793No Hit
CTCAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGG357550.20384929963754678No Hit
GAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAAC347880.19833616097863171No Hit
TTGAAACCCAGAGGCAGAATGAAAGTGAAGACGGCTTCGGCCGCTGAGGC343590.1958903114598369No Hit
CGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAAGGTG335830.19146611745847386No Hit
CGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA323320.18433381501555482No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA320310.18261772945574772No Hit
GTTAGTTTTACCCTACTGATGAAGTGTTGTTGCAATAGTAATTCTGCTCA314610.17936799932588054No Hit
CAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAAAC307760.17546262188911033No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTG305200.17400309397113486No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAA298660.170274456243182No Hit
CGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTCA291540.16621514422131278No Hit
AGAACTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAA291350.16610681988365053No Hit
TCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATG290980.16589587248925564No Hit
AAAGAAACTAACAAGGATTCCCTCAGTAACGGCGAGTGAAGCGGGAATAG290750.16576474302787506No Hit
CGGGAATGCAGCCCAAAAATGGTGGTAAACTCCATCTAAAGCTAAATACT287440.16387761904018025No Hit
CGGCGAGTGAAGCGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGT282510.1610668875418916No Hit
ATTAGATTAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCAT277190.15803380608734888No Hit
GGCAAGGCTACCCGCTGAATTTAAGCATATTAATAAGCGGAGGAAAAGAA276200.1574693792753193No Hit
CAGCAATGCGCCCCTTGAGATTCAGCCGGGCGGTTGTGGCGGTCCGGGGC268410.15302808143116747No Hit
CTCTTATCGTTTTTTTACTTATTGGATGAAGTGGAAGCGGACCGCAAGGT267770.1526631994516736No Hit
GACTAATCGAACTGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA265930.15161416376062875No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT263380.1501603371235829No Hit
CGGGAATAGCTCAAATTTGAAATCTCCGACGCTTGCGTCGGCGAGTTGTA261890.14931084626507377No Hit
GTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAA261450.14905998990417174No Hit
GGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC257900.14703603517416672No Hit
CGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGACGCATCG256300.14612383022543207No Hit
CAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTATA251780.14354685124525668No Hit
CTGGAACTCAGTGTACAGTTTTATCAGGTAAAGCGAATGATTAGAGGCAT240890.13733815631293145No Hit
GTTAAAACAACCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCG236450.1348067875801928No Hit
CGAACGCGAAGTTAAAGTGCCAAAGTCGACGCTCATCAGACCCCACAAAA233560.13315911739154085No Hit
CAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTATGGG233530.13314201354875205No Hit
GTTAATTCCGTTAACGAACGAGACCTTAACCTGCTAAATAGTTACGCGAA231920.13222410731908782No Hit
CGAACAACGAACTTAGAACTGGCAGTGACTAGGGGAATCCGACTGTTTAA231640.13206447145305925No Hit
ATTGGCTCTAAGCGTTGGGTCTCTCGGGCTGAGACTTGAAGCGGGTGGAG230440.13138031774150824No Hit
GTGAACTATGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCT229340.1307531768392532No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG217940.12425371657951878No Hit
CTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAG217910.12423661273673No Hit
GTGAAGACGGCTTCGGCCGCTGAGGCGAGATTCCGACTCTCGTAGCCGGA212990.12143158251937095No Hit
ACCAGGTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGAT212920.12139167355286382No Hit
GCGAGTCTTAGGGTGCTTGAAACCCAGAGGCAGAATGAAAGTGAAGACGG212210.12098688260686283No Hit
CTTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAA209220.11928219960891492No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACC203800.11619210534507629No Hit
CTCAAAGGTTCCGGGAAGGTAGGTCCGGACGCTCGCGTTCGGACTGTTAC202690.11555926316189165No Hit
CGGCAAAATAGATCCGTAACTTCGGGAAAAGGATTGGCTCTAAGCGTTGG201770.1150347453163692No Hit
CCTTAACCTATTCTCAAACTTTAAATTGGTAAGATGTCCGACTTGCTTAA197850.11279984319196933No Hit
TTTGAAAAGAGAGTTAAAAAGTACGTGAAACCGTTTGAAGGGAAACGAAT197460.11257749323571524No Hit
CCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTAT197080.11236084456039076No Hit
TGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGAATGGCTCATT196960.11229242918923565No Hit
GTTTTATCAGGTAAAGCGAATGATTAGAGGCATTAGGGTTAAAACAACCT193860.11052503210106226No Hit
CAGGCCCCGACGAGTAGGAGGGCGCAGTGGTCGTGACGCAGCCTTTGGCG191250.10903699777843887No Hit
GATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACTGCGA190060.10835854534781747No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG188810.10764588523161853No Hit
CCGGAAACGGTGTTGACGCAATGTGATTTCTGCCCAGTGCTCTGAATGTC188010.1071897827572512No Hit
GTCCAGACATAGTGAGGATTGACAGATTGAGAGCTCTTTCTTGATTCTAT185920.10599821504296655No Hit
CTGAAATCGGATTGTCCGGAGATAGGGTTTAATGCCTGGTAAAGCACCAC184520.10520003571282373No Hit
GCCGTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAAC184150.10498908831842885No Hit
CAGCAATGCGCCGGTGAAATACCACTACTCTTATCGTTTTTTTACTTATT181220.10331861300605853No Hit
GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC179940.1025888490470708No Hit
GTTTAATGCCTGGTAAAGCACCACACTTCTTGTGGTGTCCGGTGCGTTCT179890.10256034264242285No Hit
CAAAGATTAAGCCATGCATGTCTAAGTATAAGCACTAGTACTGTGAAACT178770.10192179917830858No Hit
CTCTGGTGGAGGCTCGTAGCGATTCTGACGTGCAAATCGATCGTCAAATT178700.10188189021180145No Hit
TAAAAACCAATGCGGGTTCTGCCCGGTGTCTTTGGTGATTCATAGTAACT177600.10125474930954637No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)