Bismark report for: /home/shared/8TB_HDD_01/sam/gitrepos/urol-e5/timeseries_molecular/D-Apul/output/01.20-D-Apul-WGBS-trimming-fastp-FastQC-MultiQC/1F5_R1_001.fastp-trim.fq.gz and /home/shared/8TB_HDD_01/sam/gitrepos/urol-e5/timeseries_molecular/D-Apul/output/01.20-D-Apul-WGBS-trimming-fastp-FastQC-MultiQC/1F5_R2_001.fastp-trim.fq.gz (version: v0.24.0) Bismark was run with Bowtie 2 against the bisulfite genome of /home/shared/8TB_HDD_01/sam/gitrepos/urol-e5/timeseries_molecular/D-Apul/data/ with the specified options: -q --score-min L,0,-1.0 -p 8 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 45838591 Number of paired-end alignments with a unique best hit: 29316139 Mapping efficiency: 64.0% Sequence pairs with no alignments under any condition: 7054716 Sequence pairs did not map uniquely: 9467736 Sequence pairs which were discarded because genomic sequence could not be extracted: 15 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 14680221 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 14635903 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 1360396266 Total methylated C's in CpG context: 20605660 Total methylated C's in CHG context: 1424600 Total methylated C's in CHH context: 5719187 Total methylated C's in Unknown context: 67746 Total unmethylated C's in CpG context: 193970435 Total unmethylated C's in CHG context: 229803206 Total unmethylated C's in CHH context: 908873178 Total unmethylated C's in Unknown context: 3429555 C methylated in CpG context: 9.6% C methylated in CHG context: 0.6% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 1.9% Bismark completed in 0d 3h 16m 2s