FastQCFastQC Report
Fri 3 Jan 2025
1A8_R2_001.fastp-trim.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A8_R2_001.fastp-trim.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53054030
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length15-125
%GC22

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAATCAACAAATTTTAATAATTTTTCACCAAATACCATACTTTTTA977050.18416131630339863No Hit
AAATTAAATCAAAACCCTAATACACAATACTTTACAAAAAAAATTACTTA794480.14974922734427526No Hit
AACCACCAAAACTTATCAATCTCCTCAAAATAAACAACTTTTTCTACAAA724220.13650612403996454No Hit
AAAAAAATCAACAAATTTTAATAATTTTTCAACAAATACCATACTTTTTA709990.13382395267616803No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)