FastQCFastQC Report
Fri 20 Dec 2024
2G1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2G1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12374729
Total Bases334.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA7014235.668188774073355No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4104543.3168726361603555No Hit
TGAAAATCTTTGCTCTGAGGTGGAA3941553.185160660892049No Hit
TGAAAATCTTTCTTCTGAGACGGAA2615212.1133472902719728No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1878631.5181180937376488No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC1455381.1760904016564726No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT1250281.0103493983585419No Hit
TTAACGAGTAGATAAATGAAGAGT1087210.8785727752098652No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC1034340.8358486072705108No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT707700.5718913117208466No Hit
TCCAAGACCTGAGTTACCACTGACAAGC645670.5217649614791564No Hit
CACCCCTCCCCGCACATTGACCTCTT644550.52085989115398No Hit
TCCCGTAGATCCGAACTTGTGG564180.4559130143375261No Hit
TAGTAACAATGACCTGATCTGGAGACTCC552300.44631280410261914No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA520150.4203324371790283No Hit
TCCCGTAGATCCGAACTTGT458800.370755593920481No Hit
TAACGTAAAATCTGAACAAATCTTGTTC437960.3539148210841627No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC388230.3137280824493207No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT362570.2929922748207254No Hit
CACCCCTCCCCGCACATTGACCTCTTA340390.2750686499882139No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC319330.2580500954808788No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC317450.25653087029218985No Hit
GAGGTCCGGACTTGGGGAGGGTTATC303980.24564578343493423No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT292120.2360617351701197No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC267380.2160693781657764No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC237600.19200420469813925No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGT236020.1907274090608368No Hit
TCAGCAAATATTTCCGACTGCTTGATGAGC235580.19037184571880322No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT234790.189733447900152No Hit
ACCCCTCCCCGCACATTGACCTCTT229330.18532123006491696No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC224220.1811918467062996No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT219450.1773372168392536No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA216430.17489675935529578No Hit
TCCTGTCTGAATTCAACTCTGAACCGTTGTC208270.1683026755575819No Hit
TCCAAGACCTGAGTTACCACTGACGAGC204930.1656036265521451No Hit
ACCCCTCCCCGCACATTGACCTCTTA201670.16296922542707803No Hit
TGAAAATCTTTCTTCTGAGGCGGA200590.1620964790420865No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG194030.1567953528517675No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC193970.1567468669414902No Hit
TTATGGAATTTAAGGGACATACGACGAGT190250.15374074050429712No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCC188840.15260132161278037No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT186610.15079926194747376No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT179180.1447950900581338No Hit
TGAAAATCTTTCTTCTGGGGCGGAA178590.14431831194040692No Hit
CGGACCTCGGATCAGCCTG178180.143986991553512No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC175220.14159501997983148No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT174790.14124753762284412No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA172110.1390818336304577No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC169630.1370777493389956No Hit
TTTACGTGTTGAGATGCCTTTATCTGAAA166320.13440294328869748No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC160740.12989375363290784No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC159670.1290290882329625No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACATT159400.12881090163671463No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT149880.1211178038727151No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC144670.11690761066363553No Hit
CACCCCTCCCCGAACATTGACCTCATA142740.11534798054971547No Hit
CGGACCTCGGATCAGCCT140900.11386107930121137No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT140480.1135216779292702No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC135920.10983674874819481No Hit
TGAAAATCTTTGCTCTGAGGTGGA134900.10901248827348056No Hit
CCTGACCTCTGATCAGACGTGA133880.10818822779876634No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT133740.1080750940081193No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACTC133420.1078165024866403No Hit
TGAGGTAGTGATTTGTGTAGCT132690.10722659057826639No Hit
CACCCCTCCCCGCACATTGACCTCTA132690.10722659057826639No Hit
GCACTGTGGTTCAGTGGTAGAATTCT126820.10248305235613644No Hit
AGTCTTGAAGAAAACTTGATTCTGATT125840.10169111582160707No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA125270.10123049967397267No Hit
TTGACTGTTTTGAAGTATCGATTGAACTGG124460.10057593988522902No Hit
TTGTCTACTTGTTCATCACAGAACCTCAAGT123950.10016380964787189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)