FastQCFastQC Report
Fri 20 Dec 2024
2F1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2F1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10783211
Total Bases277.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTC6096175.653390256390234No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2902752.6919161648603556No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1813811.6820685415503787No Hit
ACTGGTGGTTCAGTGGTAGAATTCTC1424861.321368931758824No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC844330.7830042461378155No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT744660.6905735221169279No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT549950.510005785846164No Hit
ACTGTGGTTCAGTGGTAGAATTCTC512040.4748492819068457No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT420550.390004424470596No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA402580.3733396295407741No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC286540.26572789867507923No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC283910.26328892201033627No Hit
GTTCGCGCTAGTCCCCGCA258740.23994708069794796No Hit
CGATTCGTCGGTGACCGTC258330.2395668600011629No Hit
CTGGTGGTTCAGTGGTAGAATTCTC249350.23123909937401763No Hit
CTGGGAATACCCCGTGTCGTAGGCTT225900.20949233025302016No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT218320.2024628842002628No Hit
TGCAACGGCGTTCGTGCTGGGGACA202330.18763427702564664No Hit
CTTGCAACGGCGTTCGTGCTGGGGACA201900.18723550897779892No Hit
GGTCGAACTTGATCGTTTAGAGGAAGT180150.16706526469712962No Hit
GCTTGACTCTAGTCTGACTCTGTCA165520.153497877394776No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTT160470.1488146712514482No Hit
CGGAGGAGGTGATCGCATTCTGAGCGGT159650.14805422985787814No Hit
TTGCAACGGCGTTCGTGCTGGGGACA157000.14559670584207246No Hit
TCCCGTAGATCCGAACTTGTGG154220.14301862404435933No Hit
CGATTCGTCGGTGACCGT148120.13736168197024057No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC141410.13113904568870996No Hit
GCTTGTCGTGTTCGGGAAAGCCGGTCGAACT137810.1278005224974268No Hit
CCGGTCGAGATAGTCAAACATGACCCTGT137760.12775415411977006No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTC136770.1268360602421672No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT131620.12206011734352597No Hit
CTTGTCGTGTTCGGGAAAGCCGGTCGAACT130810.12130894962548726No Hit
GGTGGATTCGGACGCGGAGC127150.11791478438101602No Hit
GATTCGTCGGTGACCGTC126530.11733981649807279No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG124680.11562418652477449No Hit
GCACTGTGGTTCAGTGGTAGAATTCT121310.11249895787071215No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA117880.10931808716346181No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGA117570.10903060322199018No Hit
ATTCCGTGAACGAACGAGACCTCGCTCT115020.10666581596149793No Hit
CGGTCGAACTTGATCGTTTAGAGGAAGT112490.10431957605206836No Hit
CCGGTCGAGATAGTCAAACATGGCCCTGT110850.10279869326492823No Hit
CCGACCGTCAACGCTGCCGACTGGGC110450.10242774624367454No Hit
GTTCGCGCTAGTCCCCGC109030.10111088431822395No Hit
CAACGGCGTTCGTGCTGGGGACA108780.10087904242994039No Hit
CCGTGAACGAACGAGACCTCGCTCTCTAC108700.10080485302568967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)