FastQCFastQC Report
Fri 20 Dec 2024
2E2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2E2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11769120
Total Bases316 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC142798312.133303084682627No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT5203094.420967752899112No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC1891601.6072569571896627No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1843501.5663872914882335No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1643601.396536019685414No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1522511.2936481232241663No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT1008210.8566570822627351No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTG833030.7078099297143712No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC783030.6653258697336759No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT734950.6244731976562394No Hit
GTTCGCGCTAGTCCCCGCA723760.6149652650325598No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT677230.5754295988145248No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT661390.5619706486126406No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCT568720.48323069184441997No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT515170.43773026360509537No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTA493820.4195895699933385No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT492950.4188503473496744No Hit
GAGGTCCGGACTTGGGGAGGGT483800.41107576437320714No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGT454520.38619709884851205No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC428100.36374852155471266No Hit
GTTCGCGCTAGTCCCCGCC389200.33069592288973176No Hit
GTTCGCGCTAGTCCCCGC371610.3157500305885232No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCC363680.3090120586755849No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT326680.27757385428987047No Hit
ATGGATGAAGAAATCTTATGTAAGCT318370.2705130035210789No Hit
TCCGGATTAGCTCAGCGGTAGAGCAGT286100.24309379120953817No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG269880.22931196215180064No Hit
TCCCATATGGTCTAGCGGTTAGGATTCCT267380.22718775915276587No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC236660.20108555270062672No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC234380.199148279565507No Hit
AAAGGATTCTAATAATGGTAGTTTTG229810.1952652364832715No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGC218600.1857403102355996No Hit
TTCCTTGGTTCTACGATCCA216920.18431284582024823No Hit
GTTCGCGCTAGTCCCCGCT215650.18323375069673858No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA213340.18127098712563045No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA204260.1735558818331362No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCC187120.1589923460717539No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC182650.15519427110947973No Hit
TCCGGATTAGCTCAGCGGTAGAGCA176250.14975631143195073No Hit
ATGGATGAAGAAATCTTATGTAAGCTT167810.14258500210720937No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACT166090.14112355044387345No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC158990.13509081392661473No Hit
GCGTCGGTGGTATAGTGGTGAGCATAGC157710.13400322199110895No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT152050.12919402640129424No Hit
GAGGTCCGGACTTGGGGAGGGTT152040.12918552958929808No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC151730.1289221284174178No Hit
TACCTGGTTGATCCTGCC151100.12838682926166103No Hit
GAGGTCCGGACTTGGGGAGG143520.12194624576858763No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGC142270.12088414426907025No Hit
CGATTCGTCGGTGACCGT141190.11996648857348721No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA139900.1188703998259853No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT139850.1188279157660046No Hit
GAGGTCCGGACTTGGGGAG138800.11793575050640999No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCT135040.11474094919586171No Hit
GGCCCTGGCCCAATGGATAAGGCATCTGACT134740.11448604483597755No Hit
TGAAAATCTTTGCTCTGAGGTGGAA125610.1067284554835026No Hit
TCCTCGGTAGTATAGTGGTGAGTATCC124130.10547092730807402No Hit
GCACTGGTGGTTCAGTGGTAGAATTCCCGCC123920.1052924942561551No Hit
AGAGAACGAGGTATTGTACGCCGCAGAGT121710.10341469880500836No Hit
GAGGTCCGGACTTGGGGA121500.10323626575308943No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)