FastQCFastQC Report
Fri 20 Dec 2024
2D2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2D2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11056415
Total Bases294.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3776793.41592641014289No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC1885531.7053719492258566No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1733331.567714308842423No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1025290.9273259008457985No Hit
AAAGGATTCTAATAATGGTAGTTTTG870820.7876151537365412No Hit
TGAAAATCTTTGCTCTGAGGTGGAA825220.7463721287596387No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT814950.7370834036168142No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT779810.7053009497201399No Hit
TGAAAATCTTTCTTCTGAGGCGGAA770430.6968171871262068No Hit
ATGGATGAAGAAATCTTATGTAAGCT665310.6017411611268209No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC524860.47471083529335684No Hit
GTTCGCGCTAGTCCCCGCA501070.45319391502580175No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC459820.41588525756314326No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA449000.40609908365415015No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT417100.3772470552163608No Hit
TCCCGTAGATCCGAACTTGTGG405990.3671985901397514No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC394070.35641751869842075No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA385100.348304581548359No Hit
ATGGATGAAGAAATCTTATGTAAGCTT381080.3446686832938163No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT375110.3392691030501297No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC309110.279575251109876No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC281840.25491083683092575No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC272900.24682503324992777No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC262010.23697554767978588No Hit
TAGGCTGCAGCATAAACACCTTTGC258050.23339391656337066No Hit
CAAAAGTCCATAATAAGTTGGCTCCAAGAAC244510.2211476323925974No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT241870.21875987831498728No Hit
AAAGGATTCTAATAATGGTAGTTTTGATAC236100.21354118853172568No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC235150.21268195884470692No Hit
TAGTAACAATGACCTGATCTGGAGACTCC231700.20956159840237548No Hit
TGAAAATCTTTCTTCTGAGACGGAA224970.20347463440907382No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCC219760.19876243791500228No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT209420.18941040111102922No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCT208700.18875919545349917No Hit
TCCCGTAGATCCGAACTTGT204450.18491527316946768No Hit
AAAGGATTCTAATAATGGTAGTTTT196580.17779723355174348No Hit
GTTCGCGCTAGTCCCCGC193430.17494820880004958No Hit
GAGGTCCGGACTTGGGGA189560.1714479783908256No Hit
AAAGGATTCTAATAATGGTAGT189460.17135753316061308No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA177610.1606397733804312No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTA172510.1560270666395934No Hit
GGTGACCGCCTGGGAATACCCCGTGTCGTA171060.15471561080151205No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC168360.15227358958577442No Hit
TCGGTACTCGGTTCTTCTCTCTAAGC164610.14888189345280545No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACT164490.14877335917655043No Hit
AAAGGATTCTAATAATGGTAGTTTTGA162520.1469915881413641No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT157690.14262308352210007No Hit
AAAGGATTCTAATAATGGTAGTTTTGATACT153550.13887865099130234No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA151830.13732299303164724No Hit
GAGGTCCGGACTTGGGGAGGGTTATC151240.13678936617339346No Hit
TACCACCCCTCCCCGCACATTGACCTCTT147680.13356951597782826No Hit
AAAGGATTCTAATAATGGTAGTTTTGAT147010.13296353293540447No Hit
TCCAAGACCTGAGTTACCACTGACAAGC142290.1286945180693742No Hit
CCGGTGAATTATTCGGACTGACGCAGTGC141510.12798904527371666No Hit
GTTCGCGCTAGTCCCCGCC136010.12301455761202885No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA133540.12078056042577996No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC129450.11708135051008849No Hit
CCGGTGAATTATTCGGACTGACGCA128790.11648441199068596No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC127770.1155618706425184No Hit
AAAGGATTCTAATAATGGTAGTT126240.11417805862026706No Hit
TCGGTACTCTGTATTTCTTCCTGAGCA125010.11306558228865322No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT124570.1126676232757182No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA122320.11063260559593684No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT120860.10931210523483426No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGC118460.10714141970973412No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA115000.10401201474438143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)