FastQCFastQC Report
Fri 20 Dec 2024
2C2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15758943
Total Bases443.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA10967226.959362693297387No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5681153.605032393352778No Hit
TGAAAATCTTTCTTCTGAGACGGAA3220212.043417505856833No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1972541.2516956245098418No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1890691.1997568618656722No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1632351.0358245473697063No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA1317680.8361474497369524No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT1170830.7429622659336987No Hit
TAGTAACAATGACCTGATCTGGAGACTCC987730.6267742703301864No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC794790.5043422011235145No Hit
TGAAAATCTTTCTTCTGGGGCGGAA785680.49856135655798745No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG732150.4645933423326679No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT728440.46223912352497243No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC698160.44302463686809446No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT690310.4380433383127282No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC645190.40941197642506866No Hit
TCCAAGACCTGAGTTACCACTGACGAGC494210.31360605847739914No Hit
CACCCCTCCCCGCACATTGACCTCTT454060.2881284614075957No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT426430.2705955596133573No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC369430.2344256210584682No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT335050.21260943706694035No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC334070.21198756794792648No Hit
TGAAAATCTTTCTTCTGAGGCGGA329340.2089860976081962No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT327780.20799618350037818No Hit
TCCCGTAGATCCGAACTTGTGG319510.20274836960829162No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA316840.20105409353914158No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA311970.19796378475383786No Hit
TCACATCGAAAATCGTACATGGATTCACT282600.1793267479931871No Hit
TGTAATCTATGGTCAGGACCTCCTGTAGT281250.1784700915537292No Hit
TCCCGTAGATCCGAACTTGT272080.17265117336867072No Hit
TTGACATGATTTCCGGACTGTTGACCTGG271850.17250522449380012No Hit
TTAACGAGTAGATAAATGAAGAGT263520.1672193369821821No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC257780.16357696071367223No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT255930.16240302411145213No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA245400.15572110388368052No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC240860.1528402000057999No Hit
TCACGTGTAACTGTGCATTTCCTTGTC235450.14940722864471304No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT233220.14799215911879368No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT230450.1462344270170912No Hit
CACCCCTCCCCGCACATTGACCTCTTA223070.14155137181472133No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT222500.14118967242917244No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC221550.14058684011992428No Hit
TCCAAGGCCTGAGTTACTACTGACGAGC218130.13841664380663093No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT208050.13202027572534528No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG203810.12932973994512195No Hit
TAACGTAAAATCTGAACAAATCTTGTTC202540.128523848331706No Hit
AGGGGTTAACCGTGAGGATACAAATGAAA198300.12583331255148267No Hit
TAAGATTGTGGATTCCGTAAATAAAAAGT194860.12365042503167883No Hit
TGATCTTTCGTCGCGTTGTTGCTCGAGTG191430.12147388311513024No Hit
ATAGCATGAAGTCAAACAAGAGAACCCGCT186920.11861201604701534No Hit
TCGGCATCGATTAAGCTGTGGATGAGCTT181270.11502675020780265No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC177760.11279944346521209No Hit
TTTACGTGTTGAGATGCCTTTATCTGAAA177570.11267887700336246No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA176200.11180952935739409No Hit
TTTTACGTATCTGTTGTCTCAATCTGGAGC168200.10673304675319913No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT166590.10571140462910487No Hit
TTGGTTGAATATAGTAACAGCAAGAACAGT166460.10562891178678671No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT166100.10540047006959793No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC164480.10437248234224847No Hit
GAGGTCCGGACTTGGGGAGGGTTATC162580.10316681772375216No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAG162350.10302086884888155No Hit
TATGAGGATCTTACAGAGACTGGCTCTTA162330.10300817764237107No Hit
TCATATTCTGTTCTTTCTAACCCTGAAAGC159480.10119968071462661No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)