FastQCFastQC Report
Fri 20 Dec 2024
2C1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2C1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13442486
Total Bases370 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC9751147.253970731306694No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC4739153.525501161020365No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT3862202.8731292708804013No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1775811.3210428487706811No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC1024640.7622399606739408No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC895860.6664392285772142No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA885580.6587918335938754No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT878480.6535100724672505No Hit
TGAAAATCTTTCTTCTGAGGCGGAA856610.63724076037721No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT684500.5092064072077144No Hit
GTTCGCGCTAGTCCCCGCA592500.44076668556694054No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC521090.38764407119337896No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC497360.3699910864701663No Hit
TCCCGTAGATCCGAACTTGTGG449810.33461816512213594No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC446380.33206655376096356No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA437820.32569868400830027No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC437280.32529697259866963No Hit
TAGTAACAATGACCTGATCTGGAGACTCC436690.3248580656881473No Hit
GAGGTCCGGACTTGGGGAGGGTTATC394910.2937775051430219No Hit
TACCACCCCTCCCCGCACATTGACCTCTT392020.29162760519148023No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC383910.2855944949468424No Hit
GTTCGCGCTAGTCCCCGCC332240.24715666432533387No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC320650.23853474721863202No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT303920.22608913262026084No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT294960.21942369886046373No Hit
GTTCGCGCTAGTCCCCGC291690.21699111310214494No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT271760.2021649864467034No Hit
TCCAAGACCTGAGTTACCACTGACAAGC267620.19908519897286858No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA260310.19364721674249838No Hit
CACAACTTTCAGCGACGGATATCTCGGCT260080.19347611743839643No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC251860.18736117709179687No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA249190.18537493734417876No Hit
TGAAAATCTTTCTTCTGAGACGGAA243860.18140989694912088No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT242640.18050232672736277No Hit
ATTACCACCCCTCCCCGCACATTGACCTCTT216000.16068456385225174No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC213780.15903308361265914No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG206030.1532677809744418No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC203640.15148983603181732No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT202610.15072360871344778No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT200850.1494143270820591No Hit
TGAAAATCTTTCTTCTGGGGCGGAA200740.1493324969800973No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA198920.1479785807476385No Hit
TCCCGTAGATCCGAACTTGT193910.14425159155828765No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGC188470.140204721061268No Hit
GAGGTCCGGACCTGGGGAGGGTTATCC183190.13627687616710182No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT177340.13192500256277No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA167720.12476858819120214No Hit
TACCACCCCTCCCCGCACATTGACCTCTTT161910.12044647098758368No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGC159020.118296571036042No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG152130.11317103101316231No Hit
TCACATCGAAAATCGTACATGGATTCACT143640.1068552349617474No Hit
TACCACCCCTCCCCGAACATTGACCTCATA140340.10440033190289356No Hit
TTACCACCCCTCCCCGCACATTGACCTCTT138820.1032695886757851No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)