FastQCFastQC Report
Fri 20 Dec 2024
2B3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17445078
Total Bases487.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC15719119.010627524852568No Hit
TGAAAATCTTTCTTCTGAGGCGGAA10495056.016052206817304No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC4229302.424351441707512No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3412681.9562423280652572No Hit
TCCAAGACCTGAGTTACCACTGACAAGC2767351.5863213681245794No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT2128741.2202524975812663No Hit
TGAAAATCTTTCTTCTGAGACGGAA2120311.2154201890068934No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC1202020.6890310264018309No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1109550.6360246712568439No Hit
TAGTAACAATGACCTGATCTGGAGACTCC1001940.5743396504160084No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT932370.5344602070566838No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC905280.5189314716735574No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA877500.5030072092540945No Hit
TCCAAGACCTGAGTTACCACTGACGAGC873840.500909196278744No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT820610.4703962917219401No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT792110.4540593054384738No Hit
TGAAAATCTTTCTTCTGGGGCGGAA787000.45113011245922774No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC765590.4388573097810168No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG677200.3881897231987154No Hit
TCCCGTAGATCCGAACTTGT645670.37011585732090163No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC576400.3304083822382451No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT566880.32495125559197846No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT539000.3089696704136261No Hit
CACCCCTCCCCGCACATTGACCTCTT463720.26581709752171934No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC443230.2540716642252904No Hit
TCCCGTAGATCCGAACTTGTGG398820.22861462700252758No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC379950.21779782240010623No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC370160.2121859243048383No Hit
TTAACGAGTAGATAAATGAAGAGT368280.2111082564377184No Hit
TCCAAGACCTGAGTTACCACTGACAAGT362370.2077204813873575No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT330330.18935426943920802No Hit
TGAAAATCTTTCTTCTGAGGCGGA304570.17458792674931004No Hit
TCCAAGGCCTGAGTTACTACTGACGAGC302600.17345866839918975No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA299250.17153835597639633No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT292800.16784103802803288No Hit
GAGGTCCGGACTTGGGGAGGGTTATC292120.1674512432675853No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA287640.16488318366934215No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT278910.15987890681830141No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT273850.15697837521850003No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC259720.1488786693874341No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT226730.1299678912298357No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC220740.12653425797236334No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC220040.12613299866013783No Hit
AGGGGTTAACCGTGAGGATACAAATGAAA219680.12592663672813614No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA219110.12559989700246685No Hit
TTTACGTGTTGAGATGCCTTTATCTGAAA218310.125141314931352No Hit
TGACTGCTGACATGAAGAAGAAGCTCAAGC217890.12490055934401668No Hit
AGCACTGATGATTGAATACTTTATGATG208500.1195179522843062No Hit
CACCCCTCCCCGCACATTGACCTCTTA199080.11411814839692891No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC197920.11345320439381239No Hit
TGTAATCTATGGTCAGGACCTCCTGTAGT194430.11145264010857389No Hit
TCCAAGGCCTGAGTTACCACTGACGAGC191350.10968709913478174No Hit
TCCAAGACCTGAGTTACCACTGACGAGCAGT186280.1067808352590914No Hit
TCACATCGAAAATCGTACATGGATTCACT182490.10460830269718485No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT180230.10331280834628542No Hit
CACAACTTTCAGCGACGGATATCTCGGCT178150.10212049496138682No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA178020.10204597537483066No Hit
ATAGCATGAAGTCAAACAAGAGAACCCGCT176590.10122625992271288No Hit
GACCAGTGCACCCGTGTTTTGAATATGAATT174760.10017725343503767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)