FastQCFastQC Report
Fri 20 Dec 2024
2B2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2B2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11611826
Total Bases311.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC6319025.441883128458866No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC4892874.213695589306971No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2252431.9397724354464148No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT2065911.7791430908454882No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1799121.5493859449840188No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1105390.9519519152284921No Hit
TGAAAATCTTTCTTCTGAGGCGGAA968280.8338740177470796No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA627960.5407934979390838No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC492840.4244293705399995No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC490050.4220266476607555No Hit
TCCCGTAGATCCGAACTTGTGG475780.4097374521457693No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT401460.34573373731228835No Hit
TAGTAACAATGACCTGATCTGGAGACTCC321950.27726044120881593No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC296190.25507616114812603No Hit
GTTCGCGCTAGTCCCCGCA290850.25047740122871287No Hit
CGGAGGAGGTGATCGCATTCTGAGCGGT281870.24274390608333263No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA255660.22017209007437763No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA250450.21568528498446324No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA225900.1945430460291086No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC221070.19038349351772924No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT212770.18323560824972746No Hit
TCCCGTAGATCCGAACTTGT212710.18318393678995878No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC206500.17783594070389963No Hit
TGAAAATCTTTCTTCTGGGGCGGAA166830.1436724938868357No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC157590.13571508908245783No Hit
TCCAAGACCTGAGTTACCACTGACAAGC156210.13452664550777801No Hit
CTGGGAATACCCCGTGTCGTAGGCTT155330.1337687974311706No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA144190.12417512973411761No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT143550.12362396749658495No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC140810.12126430416714823No Hit
TCGGTACTCTGTATTTCTTCCTGAGCA140750.12121263270737953No Hit
GAGGTCCGGACTTGGGGAGGGTTATC138440.11922328150628507No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC136060.11717364693546045No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA133410.11489149079567675No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC128070.11029273087626355No Hit
CGATTCGTCGGTGACCGTC126120.10861340843378123No Hit
TACCACCCCTCCCCGCACATTGACCTCTT123610.1064518190334578No Hit
CGATTCTTTGAACGCAAATGGCGCTCGTCT122040.10509974916951047No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT119430.10285204066957254No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC118130.10173249237458433No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC117720.10137940406616495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)