FastQCFastQC Report
Fri 20 Dec 2024
1H8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7090647
Total Bases197.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC6112368.62031349184355No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2486963.507380920246065No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1060651.495843750224768No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA765351.0793796391217896No Hit
TGAAAATCTTTGCTCTGAGGTGGAA570270.8042566496400116No Hit
TGAAAATCTTTCTTCTGAGGCGGAA477480.6733941204519136No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT440390.6210857767986476No Hit
TCCAAGACCTGAGTTACCACTGACAAGC417610.5889589483159999No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT417450.5887332989500111No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT353250.4981914908470271No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC315190.4445151479124543No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC313090.44155349998385196No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT303050.4273940022680582No Hit
TAGTAACAATGACCTGATCTGGAGACTCC276350.3897387643186863No Hit
TCCCGTAGATCCGAACTTGTGG265650.37464846296818893No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG218800.3085755079896094No Hit
TGAAAATCTTTCTTCTGGGGCGGAA207220.292244135126174No Hit
TTATGGAATTTAAGGGACATACGACGAGT200380.2825976247301551No Hit
AAAGGATTCTAATAATGGTAGTTTTG185170.26114683187584997No Hit
TCCCGTAGATCCGAACTTGT178790.25214906340704873No Hit
TCACATCGAAAATCGTACATGGATTCACT175280.24719888044067068No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT173290.24439236645118564No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC171410.24174098640081784No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT170930.24106403830285164No Hit
TGAAAATCTTTCTTCTGAGACGGAA161690.2280327874170016No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT146840.20708970563617113No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC137260.193578949847595No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT133960.18892493167407715No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA133910.18885441624720564No Hit
TCCAAGACCTGAGTTACCACTGACGAGC130870.18456707829341948No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC118670.16736131413677766No Hit
GTTCGCGCTAGTCCCCGCA117690.16597921177009659No Hit
TCCAAGACCTGAGTTACCACTGACAAGT107580.15172099245668272No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT105030.1481247056862371No Hit
GTTCGCGCTAGTCCCCGC102440.1444720065742943No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC101850.14363992453721078No Hit
CACCCCTCCCCGCACATTGACCTCTT99790.14073468895010569No Hit
GAGGTCCGGACTTGGGGAGGGTTATC99750.1406782766086085No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT96610.13624990780107937No Hit
TAGTAACAATGACCTGATCTGGAGACTCT92820.13090483844422096No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA91550.1291137466016853No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA86310.12172372986555388No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT83840.1182402677781026No Hit
TTACGTATCTGTTGTCTCAATCTGGAGCC82560.11643507285019265No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT81740.11527861984950033No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT73610.1038128114401972No Hit
TTGACATGATTTCCGGACTGTTGACCTGG72320.10199351342691294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)