FastQCFastQC Report
Fri 20 Dec 2024
1H7-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H7-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7832969
Total Bases219 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2745773.5054013363259835No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC947471.209592429128725No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT729930.9318688737310208No Hit
TGAAAATCTTTCTTCTGAGGCGGAA657960.8399880045484668No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT620460.7921134374462608No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT619390.7907474164649444No Hit
TCCAAGACCTGAGTTACCACTGACAAGC501260.6399361468173818No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT356720.45540841537863863No Hit
TCCCGTAGATCCGAACTTGTGG345630.44125031006761295No Hit
TAGTAACAATGACCTGATCTGGAGACTCC332720.42476869243322674No Hit
TCCCGTAGATCCGAACTTGT305930.39056710169541076No Hit
TGAAAATCTTTCTTCTGAGACGGAA261430.33375594873412623No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA239000.3051205743313934No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC224320.2863792771297831No Hit
TGAAAATCTTTGCTCTGAGGTGGAA214040.273255262468165No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT213290.2722977711261209No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC202300.25826733132736773No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC187820.23978136515030252No Hit
TAACGAAGAAATCAATTGACTGTGCTGTCC182950.23356405470262936No Hit
AAAGGATTCTAATAATGGTAGTTTTG182070.23244059819463092No Hit
TGAAAATCTTTCTTCTGGGGCGGAA180360.2302575179347703No Hit
GAGGTCCGGACTTGGGGAGGGTTATC175800.22443597057514209No Hit
TCCAAGACCTGAGTTACCACTGACGAGC145600.18588098586883212No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA142580.18202548739820112No Hit
TCACATCGAAAATCGTACATGGATTCACT140790.17974027472852247No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG138270.17652310381925423No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT130680.16683329143776773No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT128850.16449701256318006No Hit
CAGCGACTGGTGGATCTGATCATCCAGCA128060.16348845501622694No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC121140.1546540015669665No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT114010.14555145054193372No Hit
TTAACGAGTAGATAAATGAAGAGT110500.14107039106116723No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC99230.12668248782805092No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA93630.11953321914078813No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA89400.11413296797165928No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC87210.11133709325289044No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA82790.10569427761044375No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)