FastQCFastQC Report
Fri 20 Dec 2024
1H6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13603218
Total Bases373.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA8006825.885974921522245No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4696703.4526389270538775No Hit
TGAAAATCTTTCTTCTGAGACGGAA2554731.8780335652931535No Hit
TAGTAACAATGACCTGATCTGGAGACTCC1739991.2791017537173925No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1721341.26539176244915No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT1583421.1640039878799266No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1537721.130408995871418No Hit
TCCAAGACCTGAGTTACCACTGACAAGC1481931.089396641294729No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA1138070.8366182178363972No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT832920.6122962963616403No Hit
TCCAAGACCTGAGTTACCACTGACGAGC656280.48244466860708984No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC620630.4562376343597522No Hit
CACCCCTCCCCGCACATTGACCTCTT606450.44581363027483645No Hit
TTAACGAGTAGATAAATGAAGAGT589700.4335003673395516No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT566430.41639412086169614No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC437570.3216665350801553No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT435510.3201521875191591No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC339300.24942627545923324No Hit
TGAAAATCTTTCTTCTGAGGCGGA320090.23530461689285578No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT309070.22720359256170122No Hit
TTAACGAGTAGATAAATGAAGAG301830.2218813224929572No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC283950.20873737375965012No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG280530.20622326276032626No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT280340.2060835899270305No Hit
TGGAAATCTTTCTTCTGAGGCGGAA278260.20455453996252945No Hit
TTATGGAATTTAAGGGACATACGACGAGT278190.20450308155026262No Hit
TACGATATGTCTTAATTCTCTGAATAGGC270970.1991955138850234No Hit
TCCCGTAGATCCGAACTTGTGG259330.19063871504521943No Hit
TGATTCCACTTGTCAAGAACACAAATGATA256560.18860243215980219No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC248010.1823171546614926No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT247700.18208926740716794No Hit
TTTACGTGTTGAGATGCCTTTATCTGAAA245500.18047200302163796No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA234730.1725547587342936No Hit
AGGGGTTAACCGTGAGGATACAAATGAAA230950.16977600447188304No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA230440.1694010931825102No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA229770.1689085626650988No Hit
TACTAGACGTGATACACTCAAGGAAGACC224750.16521825938538953No Hit
TCCCGTAGATCCGAACTTGT224230.1648359968942643No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC221550.1628658748246187No Hit
GTATCAACGATTTAAGCCTAAAGACTCTT221450.16279236280709464No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT213510.15695550861568194No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGT209360.15390475988843227No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT197600.14525974662759944No Hit
TCACATCGAAAATCGTACATGGATTCACT196080.1441423639612333No Hit
TACTAGACGTGATACACTCAAGAAAGACC191440.14073140634811557No Hit
CACCCCTCCCCGCACATTGACCTCTTA189880.13958461887473977No Hit
CGGACCTCGGATCAGCCTG185680.13649711413872806No Hit
GAGGTCCGGACTTGGGGAGGGTTATC180470.13266713802572305No Hit
TCCAAGGCCTGAGTTACCACTGACAAGC178260.13104252243844067No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT156020.11469344974108332No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT154150.11331877501338286No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC148800.10938588207584411No Hit
TTTGATCTTATCATATGTACATATCTGATG144420.10616605570828903No Hit
TTAACGAGTAGATAAATGAAG143240.10529861390150479No Hit
TCTTTTATGAGTGGAACCTCTCGACCA141380.10393129037555672No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG140840.10353432548092666No Hit
TACAAAAACAAGATGAGTGCAGG140580.10334319423536402No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC137340.10096140486758354No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)