FastQCFastQC Report
Fri 20 Dec 2024
1H12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6805816
Total Bases187.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC111477416.379725810982844No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2745214.033623594878263No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1497442.200235798323081No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1342081.9719604526481467No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1192681.75244232286033No Hit
AAAGGATTCTAATAATGGTAGTTTTG669120.9831591097966799No Hit
TGAAAATCTTTCTTCTGAGGCGGAA527440.7749842193794249No Hit
TGAAAATCTTTGCTCTGAGGTGGAA519610.7634793535411477No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT379910.5582137395427675No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC300450.44146065659136247No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC299580.4401823381648872No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC263140.3866398974053956No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC244600.3593984909377509No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA230670.33893070279890025No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT219080.3219011504278106No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC208990.3070755953437472No Hit
AAAGGATTCTAATAATGGTAGTTTT192240.28246429230528713No Hit
TGAAAATCTTTCTTCTGAGACGGAA185560.2726491577203968No Hit
TCCCGTAGATCCGAACTTGTGG183850.2701366008131868No Hit
TGAAAATCTTTCTTCTGGGGCGGAA173000.2541943537703635No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC164710.2420135954307316No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA161670.2375468275956917No Hit
TCCAAGACCTGAGTTACCACTGACAAGC158320.2326245669879997No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT153820.22601257512692088No Hit
AAAGGATTCTAATAATGGTAGT150200.2206935950075641No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT148560.21828389130708206No Hit
TAGTAACAATGACCTGATCTGGAGACTCC134270.1972871438193451No Hit
GTTCGCGCTAGTCCCCGCA122490.17997841845856544No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC115270.16936984485034565No Hit
ATGGATGAAGAAATCTTATGTAAGCT114410.16810621973911724No Hit
TCCCGTAGATCCGAACTTGT106170.15599892797571957No Hit
GTTCGCGCTAGTCCCCGC104440.15345698443801595No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC101040.14846125725408973No Hit
TTATGGAATTTAAGGGACATACGACGAGT87800.12900730786727116No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA78960.116018417189063No Hit
AAAGGATTCTAATAATGGT78500.11534252468770828No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC78190.11488703191505618No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT77900.11446092577289776No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC77880.11443153914240409No Hit
AAAGGATTCTAATAATGGTAGTTTTGAT77030.11318260734642252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA71850.10557147004855846No Hit
AAAGGATTCTAATAATGGTAGTTTTGA69280.10179528803012013No Hit
ATGGATGAAGAAATCTTATGTAAGCTT68910.10125163536598698No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)