FastQCFastQC Report
Fri 20 Dec 2024
1H11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1H11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5914953
Total Bases160.8 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2011843.401278082852053No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT924701.5633260315001656No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC830421.4039333871291961No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT668631.1304062771082035No Hit
TGAAAATCTTTCTTCTGAGGCGGAA492190.8321114301330881No Hit
TCCCGTAGATCCGAACTTGTGG444030.7506906648286132No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT391030.6610872478614792No Hit
TAGTAACAATGACCTGATCTGGAGACTCC333930.5645522458082084No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC318590.538617973803004No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA311240.5261918395632222No Hit
GTTCGCGCTAGTCCCCGCA265440.448760962259548No Hit
TCCAAGACCTGAGTTACCACTGACAAGC239560.40500744469144556No Hit
TCCCGTAGATCCGAACTTGT220900.3734602793969791No Hit
CTGGTATGATGATTGAAACTTTCGTTCTTC204930.346460910171222No Hit
TGAAAATCTTTCTTCTGAGACGGAA198590.3357423127453422No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT196800.33271608413456544No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC187280.31662128169065756No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC182160.30796525348553067No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT171050.28918234853260877No Hit
TACCACCCCTCCCCGCACATTGACCTCTT157020.2654628024939505No Hit
TCACATCGAAAATCGTACATGGATTCACT154960.261980103645794No Hit
GAGGTCCGGACTTGGGGAGGGTTATC150850.25503161225456905No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC140980.23834508913257638No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT136310.23044984465641571No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA134350.22713620885914054No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA132830.2245664504857435No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC132610.22419451177380445No Hit
TAGTAACAATGACCTGATCTGGAGACTCT132370.22378876045168913No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT130770.22108375163758698No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG129630.21915643285753916No Hit
GTTCGCGCTAGTCCCCGCC122320.20679792383810996No Hit
TGAAAATCTTTCTTCTGGGGCGGAA120530.20377169522733315No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA114880.19421963285253493No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT98550.1666116366436048No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC97120.16419403501600097No Hit
GTTCGCGCTAGTCCCCGC90100.15232580884412775No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC89050.1505506468098732No Hit
TCCCGTAGATCCGAACTTGTGA83180.14062664572313593No Hit
TCCAAGACCTGAGTTACCACTGACGAGC81180.13724538470550823No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC77860.13163249141624625No Hit
GTTCGCGCTAGTCCCCGCT76410.1291810771784662No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA76210.1288429510767034No Hit
TTGACATGATTTCCGGACTGTTGACCTGG75810.12816669887317786No Hit
TCCAAGACCTGAGTTACCACTGACAAGT74280.12558003419469269No Hit
GCACTGTGGTTCAGTGGTAGAATTCT73540.12432896761817042No Hit
TACCTGGTTGATCCTGCC72730.1229595569060312No Hit
GGGAATACCCCGTGTCGTAGGCTT72610.12275668124497353No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT72560.12267214971953286No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT72380.12236783622794635No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT69280.11712688165062342No Hit
ATTACCACCCCTCCCCGCACATTGACCTCTT68560.11590962768427746No Hit
TGATTCCACTTGTCAAGAACACAAATGATA68420.1156729394130435No Hit
CACCCCTCCCCGCACATTGACCTCTT66120.11178448924277165No Hit
TACCACCCCTCCCCGCACATTGACCTCTTT65990.11156470727662587No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT64900.10972192002201876No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT61720.10434571500399074No Hit
TACCACCCCTCCCCGAACATTGACCTCATA61380.10377090063099402No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)