FastQCFastQC Report
Fri 20 Dec 2024
1G5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1G5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13186143
Total Bases369.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA5451784.134476624438245No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT3389502.570501472644427No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2567011.9467481886098155No Hit
TGAAAATCTTTCTTCTGAGACGGAA1946081.4758523398388748No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1309080.9927694550256281No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1194790.9060951333532482No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA1024500.7769519866423411No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT789090.5984236633866324No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT774860.5876320315955924No Hit
TAGTAACAATGACCTGATCTGGAGACTCC755770.5731547124887088No Hit
TCCCGTAGATCCGAACTTGTGG624610.4736866572734726No Hit
TCCAAGACCTGAGTTACCACTGACAAGC573390.434842849800734No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT557930.42311842060259774No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT530220.40210393592728366No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT484390.3673477528645033No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC393510.29842691680197914No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC391710.2970618474257408No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA388340.29450613420467225No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT361680.2742879399988306No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG314450.23847003631008706No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC305080.2313640918348906No Hit
TCCCGTAGATCCGAACTTGT300610.22797416955056532No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA299660.22725371626866175No Hit
TTAACGAGTAGATAAATGAAGAGT290760.22050420657503864No Hit
TCACATCGAAAATCGTACATGGATTCACT282720.21440689669450724No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT280810.2129584064119432No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT267070.20253837683999026No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC236900.17965829735048378No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT229730.1742207710018009No Hit
CACCCCTCCCCGCACATTGACCTCTT222750.16892733530949877No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC217290.16478662486824236No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC214500.16267076733507288No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA208760.15831771276862386No Hit
TTATGGAATTTAAGGGACATACGACGAGT204440.15504154626565175No Hit
TAGTAACAATGACCTGATCTGGAGACTCT188800.14318061012989167No Hit
TGAAAATCTTTCTTCTGAGGCGGA183160.13890339275101143No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC178020.13500536130997517No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA177780.13482335205981005No Hit
TTGACATGATTTCCGGACTGTTGACCTGG168130.127505063459421No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT168080.1274671448656366No Hit
TGTTGCTGGCAAAAGTAGAAATCTGGGAGT166460.12623858242702207No Hit
TTTGTCTGATTTTGAGATTTTTCTCGTC166260.12608690805188447No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC162050.12289416245523804No Hit
TCCAAGACCTGAGTTACCACTGACGAGC161230.1222722975171739No Hit
TGTTGTGTGTTCTTCTGGTCTTGACGAGT157570.11949665645215589No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACC157140.11917055654561005No Hit
GAGTAACTCAGGATTTGCAAGAGCAATAGA157060.119109886795555No Hit
TGAGACCTAGGAATAATAATGGTAGAAGGG153850.11667551307459657No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA152910.11596264351144986No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC151500.1148933391667298No Hit
ATAGCATGAAGTCAAACAAGAGAACCCGCT145440.11029760560006062No Hit
TGAGACCTAGGAATAATAATGGTAGAAGG144450.1095468174431295No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT140810.10678634381562524No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA139310.10564878600209325No Hit
GCACTGTGGTTCAGTGGTAGAATTCT138300.1048828304076484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)