FastQCFastQC Report
Fri 20 Dec 2024
1F8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7066753
Total Bases193.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC112521715.922687548298347No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC5416857.665260127246559No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT2246893.1795224765886116No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1747742.473186766256016No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1066171.50871270016088No Hit
TGAAAATCTTTCTTCTGAGGCGGAA994021.406614890884116No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC888101.25672992957303No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC820701.161353736291618No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA427980.6056246765664514No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC392410.5552903858391541No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC370090.5237058660462592No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC338880.47954131126416893No Hit
GTTCGCGCTAGTCCCCGCA228130.32282152779359913No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT215180.30449627997469275No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC198560.28097769937621986No Hit
TGAAAATCTTTCTTCTGGGGCGGAA195110.27609568354801706No Hit
TCCCGTAGATCCGAACTTGTGG189210.26774672894326434No Hit
GTTCGCGCTAGTCCCCGC169440.23977065563208447No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT168950.23907726787677452No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGG154440.2185444998572895No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT147930.20933234825102845No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC143790.20347392925718502No Hit
TAGTAACAATGACCTGATCTGGAGACTCC138780.1963843932284035No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA135030.19107785428470475No Hit
CACAACTTTCAGCGACGGATATCTCGGCT130230.1842854844367703No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA120530.17055923703573622No Hit
TCCAAGACCTGAGTTACCACTGACAAGC120220.1701205631497238No Hit
TACCTGGTTGATCCTGCC117270.16594608584734744No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG113560.1606961499857148No Hit
TCCCGTAGATCCGAACTTGT106450.15063495214846195No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGC103580.14657368101021784No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA92610.13105028575358443No Hit
TCCTCGGTAGTATAGTGGTGAGTATCCC89030.1259843099086667No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC87640.12401735280686901No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC84590.11970136779932736No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC79900.11306465642707479No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA78080.11048921619306633No Hit
GAGGTCCGGACTTGGGGAGGGTTATC75650.10705057895754953No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA75220.10644209582533874No Hit
CGATTCGTCGGTGACCGT74160.10494211415058656No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)