FastQCFastQC Report
Fri 20 Dec 2024
1F4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10897535
Total Bases299.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3219432.9542736040765183No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2206872.0251093481232223No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1808701.6597331414856662No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1470011.3489380855395279No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT1020770.9366980697928475No Hit
AAAGGATTCTAATAATGGTAGTTTTG957370.8785197753436901No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC795790.7302477119825722No Hit
TCCAAGACCTGAGTTACCACTGACAAGC701180.6434299132785534No Hit
TCCCGTAGATCCGAACTTGTGG691270.6343361136256961No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT612530.5620812413082408No Hit
CACCCCTCCCCGCACATTGACCTCTT572930.52574274824536No Hit
TGAAAATCTTTCTTCTGAGACGGAA523760.48062245269228315No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT521900.4789156446847842No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT486140.44610088428254646No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC410890.377048571075936No Hit
TCCCGTAGATCCGAACTTGT388240.3562640542104247No Hit
TAGTAACAATGACCTGATCTGGAGACTCC374570.3437199329940211No Hit
GAGGTCCGGACTTGGGGAGGGTTATC358870.32931300518878814No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA358810.32925794686596555No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT358060.3285697178306837No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG292730.26862038066406757No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT282700.25941646436556526No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC281440.2582602395862918No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT262930.24127474699553614No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA262510.2408893387357783No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC259440.23807218788469134No Hit
TACCACCCCTCCCCGCACATTGACCTCTT257880.23664067149130516No Hit
TTAACGAGTAGATAAATGAAGAGT255430.23439245664271785No Hit
TTATGGAATTTAAGGGACATACGACGAGT247990.22756522461272205No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT239020.21933400535075134No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC237420.21786578340881677No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT237220.21768225566607494No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC223910.2054684843866067No Hit
TCCAAGACCTGAGTTACCACTGACGAGC222770.20442237625297832No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT213360.19578739595697559No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC210250.19293353955734027No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA208890.19168555090669587No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA202020.18538137294351428No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC196130.17997648091976765No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCC195280.1791964880131149No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT193680.17772826607118034No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA190410.17472758747735154No Hit
CACCCCTCCCCGCACATTGACCTCTTA180620.16574390447013934No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT176600.16205499684102873No Hit
GCACTGTGGTTCAGTGGTAGAATTCT160530.14730854271172333No Hit
ATTACCACCCCTCCCCGCACATTGACCTCTT148130.13592982266173037No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACATT140860.12925858921306516No Hit
ACCCCTCCCCGCACATTGACCTCTT136160.12494568725863234No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC135930.12473463035447925No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA133260.12228453498887593No Hit
CCACCCCTCCCCGCACATTGACCTCTT133160.12219277111750501No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC131710.12086219498262679No Hit
ATAGCATGAAGTCAAACAAGAGAACCCGCT127840.11731093316057256No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC122550.11245662436505137No Hit
TCCTGTCTGAATTCAACTCTGAACCGTTGTC121420.11141969261856007No Hit
GCCAAATGATGAAATGTGGTGAGAACTGAGA121330.11133710513432625No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC121000.11103428435880223No Hit
TACCACCCCTCCCCGCACATTGACCTCTTT119400.10956606241686766No Hit
AAAGGATTCTAATAATGGTAGT117480.10780419608654616No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA117370.10770325582803816No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC116800.10718020176122398No Hit
CACCCCTCCCCGCACATTGACCTCT116770.1071526725998127No Hit
GAGGTCCGGACTTGGGGAGGGTTAT116490.10689573375997415No Hit
TCTAAGGCCTGAGTTACCACTGACAAGCAGT113340.10400517181179048No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC113220.10389505516614538No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG112200.10295906367816209No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACT112060.10283059425824281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)