FastQCFastQC Report
Fri 20 Dec 2024
1F11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1F11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7439586
Total Bases197.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA6930779.316069469457037No Hit
TGAAAATCTTTCTTCTGAGACGGAA2565113.4479203547079096No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1782292.3956843835127386No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1523622.0479903048368553No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT814951.0954238582630809No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC739400.9938725084971126No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA672480.9039212665866084No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT642950.8642281976443313No Hit
TCCAAGACCTGAGTTACCACTGACAAGC636150.8550879040849854No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG436060.5861347661012319No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC384440.5167491846992561No Hit
TCCCGTAGATCCGAACTTGTGG350270.4708192095635429No Hit
GCACTGTGGTTCAGTGGTAGAATTCT339370.4561678566522384No Hit
TTAACGAGTAGATAAATGAAGAGT337020.4530090787309939No Hit
TAGTAACAATGACCTGATCTGGAGACTCC304000.4086248885354642No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC295640.3973877041007389No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC274810.3693888342711543No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT254390.3419410703767656No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC233030.313229795313879No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA224030.3011323479559212No Hit
TGAAAATCTTTCTTCTGAGGCGGA214600.2884569114464165No Hit
TCCCGTAGATCCGAACTTGT214100.2877848310376411No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC211960.2849083268880822No Hit
TCCAAGACCTGAGTTACCACTGACGAGC202270.2718834085660143No Hit
CACCCCTCCCCGCACATTGACCTCTT161770.2174448954552041No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC160180.21530767975529824No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC149760.20130152403641816No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC139320.1872684851011871No Hit
TTGGTTGAATATAGTAACAGCAAGAACAGT138870.18666361273328919No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA133510.17945891075121653No Hit
CACAACTTTCAGCGACGGATATCTCGGCT129120.1735580447621682No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT116720.15689045062453744No Hit
TTAACGAGTAGATAAATGAAGAG116490.15658129363650075No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT112580.15132562483987685No Hit
TTGGTTGAATGTAATAACAGCGAGAACAGT111030.149242175572673No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC111010.149215292356322No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA105670.14203747359060034No Hit
CGGACCTCGGATCAGCCTG105300.1415401340881065No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC103050.13851577224861705No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACC100620.13524946146196845No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT97780.131432044740124No Hit
TCACATCGAAAATCGTACATGGATTCACT96330.1294830115546752No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG93690.12593442699634091No Hit
TGAGACCTAGGAATAATAATGGTAGAAGG92630.124509616529737No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC91770.12335363822664326No Hit
GAGGTCCGGACTTGGGGAGGGTTATC90480.12161967077200264No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCA88830.1194018054230437No Hit
CACCCCTCCCCGCACATTGACCTCTTA88430.11886414109602336No Hit
TCAGTCACAAGGTATTGCTCAGCAACTCC88250.1186221921488642No Hit
AGGGGTTAACCGTGAGGATACAAATGAAA84360.11339340656859132No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG83080.11167288072212621No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT83080.11167288072212621No Hit
CGGACCTCGGATCAGCCT79190.10644409514185332No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC78320.10527467523058406No Hit
CGGACCTCGGATCAGCCTGGA74860.10062387880185805No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)