FastQCFastQC Report
Fri 20 Dec 2024
1E9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7877518
Total Bases210.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2344072.975645374596415No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1095031.3900698164066396No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1020071.2949129408526898No Hit
TCCCGTAGATCCGAACTTGTGG494450.6276723201394145No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT479610.6088338992053081No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT464270.5893607605847425No Hit
GTTCGCGCTAGTCCCCGCA385640.48954505721218283No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA355950.45185552099024084No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC261200.3315765194062394No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT256370.32544514655504436No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC243840.3095391213323791No Hit
TGAAAATCTTTCTTCTGAGGCGGAA236170.2998025520220963No Hit
TCCCGTAGATCCGAACTTGT220810.2803040246940724No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC205040.2602850288631521No Hit
GGGCCTGTAGTTCAATTGGTTAGAGCACC204440.25952336763940115No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT195280.24789533962347024No Hit
TCCAAGACCTGAGTTACCACTGACAAGC191200.24271604330196392No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG178020.22598488508690173No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC167040.21204648469225965No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA165510.2101042485716948No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC150800.19143085423606776No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT147730.1875336876412088No Hit
GTTCGCGCTAGTCCCCGC144900.18394118553585026No Hit
TACCACCCCTCCCCGCACATTGACCTCTT127740.16215767453657357No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC122350.15531541787654435No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCC114730.1456423203349075No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCT108980.13834306694062776No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC106820.1356010865351244No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA103950.1319578070148491No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT100670.1277940589916773No Hit
CACCCCTCCCCGCACATTGACCTCTT100350.1273878396723435No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT98270.1247474140966736No Hit
GTTCGCGCTAGTCCCCGCC98240.12470933103548604No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT98010.12441736089971486No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA97420.12366839402969312No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT95400.12110413457639829No Hit
GAGGTCCGGACTTGGGGAGGGTTATC90850.11532820362962039No Hit
TCGGTACTCGGTTCTTCTCTCTAAGC90780.11523934315351611No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC88630.11251005710174194No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT86980.11041548873642688No Hit
TCCCGTAGATCCGAACTTGTGA86850.11025046213794751No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT86590.10992040894098878No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT86540.10985693717234288No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA85570.10862558486061219No Hit
TAGTAACAATGACCTGATCTGGAGACTCC84060.10670873744750567No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT82690.1049696109866077No Hit
AAAGGATTCTAATAATGGTAGTTTTG80430.10210068704381253No Hit
GTTCGCGCTAGTCCCCGCT80390.10204990962889579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)