FastQCFastQC Report
Fri 20 Dec 2024
1E5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8005903
Total Bases207 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4108485.131813363214618No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1421311.7753275301986546No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1055941.3189517784564715No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1011441.2633677924901163No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT738170.9220321555232434No Hit
CGGAATGTAGCGCAGTTTGGTAGCGTGCGTG482090.6021681751577554No Hit
GCGGTCATGGTGTAGTGGTATCATAATC408110.5097613598366105No Hit
TCCATCGTAGTCTAGGTGGTTAGGATCTCCG391230.48867691751948533No Hit
TGCGGGATAGAGCAGGCTGGTAGCTCGCAAG377740.47182685076249364No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC307860.38454125662027133No Hit
TGCGGAGCTCCCATCCGTCGGCTAACGACT299780.3744487036627848No Hit
GCGTCGGTGGTCTAGTGGTAGAATAGCTCC274110.342384862769384No Hit
GAAATTCTTGGATTTGTGGAAGGCGAA269440.33655166693875754No Hit
GCGTCGGTGGTCTAGTGGTAGAATAGCTCCC248640.31057083754324777No Hit
TCCATCGTAGTCTAGTTGGTTAGGATCTCCG224950.28098017175576573No Hit
TGAAAATCTTTCTTCTGAGACGGAA208770.2607700842740662No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCG206890.2584218170017798No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA193750.24200892766250104No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT192890.2409347202932636No Hit
TTGAACCCTGGCGTTTAGAGGATGGAGA192200.240072856241201No Hit
TCCAAGACCTGAGTTACCACTGACAAGC159480.199203013076726No Hit
CGGAATGTAGCGCAGTTTGGTAGCGTGCG157800.19710456147170405No Hit
GAGGTTCGGCCCGTATTCTTTACGTTTCT150660.18818614215036078No Hit
AAACTTAACCTTAGTTCGGATTATAGACT150190.18759907533228917No Hit
AAAACTTAACCTTAGTTCGGATTATAGACT136150.17006201549032007No Hit
GCAGCACTGGTGTAGTGGTAGCGCTTTAGC131410.16414138417615104No Hit
AAAACTAAGTGGAAGTCCGAACCGACTAATG129100.16125601321924585No Hit
GAACAGCCGACTCAGAACT123730.15454846255319357No Hit
TTTGACCTGACGGCCGACGAGGCTACCCGCT120580.15061386579377742No Hit
GATCTATATCTGAGGTGTGCCTTAAT118300.14776596718696192No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC113060.14122079670463156No Hit
GCGGTCATGGTGTAGTGGTATCATAAT112620.14067120223664964No Hit
AAATTCTTGGATTTGTGGAAGGCGAA112370.140358932652569No Hit
GAACAGCCGACTCAGAACTG110800.13839787966454253No Hit
AAGCAGGACTGGCGATGTGGGATG107580.13437584742158382No Hit
GCGGGATAGAGCAGGCTGGTAGCTCGCAAG106990.13363889120315348No Hit
CGGAATGTAGCGCAGTTTGGTAGCGTGCGT106750.13333911240243607No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC104060.12997909167772828No Hit
GCGGGATAGAGCAGGCTGGTAGCTCGCAAGG102300.12778071380580056No Hit
TGTGTCGTGAATTGCAGACCTCCGTG99310.12404596958019601No Hit
GCAGTACTGGTGTAATGGTAGCGCCTTAGCC98430.12294678064423213No Hit
GAAATTCTTGGATTTGTGGAAGGCGAACA95170.11887478526782051No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC90440.11296664473701466No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT89680.11201734520140952No Hit
TCTTCAGTAGCTCAAGGGTAGAGCAAACGG88140.11009376456347272No Hit
TCGGGCCCGAGTGGGCTGGTTCTGG87870.10975651341266564No Hit
TCGAGTCAGGCAATTGGAATG86740.1083450548926211No Hit
TGAGGTTCGGCCCGTATTCTTTACGTTTCT86660.10824512862571531No Hit
TTGAACATCCGGGGGAGCGAA80490.10053831529060495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)