FastQCFastQC Report
Fri 20 Dec 2024
1E3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17188214
Total Bases471.1 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA12730797.406697403232237No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC5908623.43759974131111No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC4699162.733943154303292No Hit
TGAAAATCTTTCTTCTGGGGCGGAA2456171.4289850010012675No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA1820351.0590687316320357No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1766351.0276518549280338No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1448040.8424610026382031No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1280270.7448534210709734No Hit
TCCCGTAGATCCGAACTTGTGG972890.5660215773436379No Hit
TTAACGAGTAGATAAATGAAGAGT795920.46306149085646714No Hit
TAGTAACAATGACCTGATCTGGAGACTCC764690.4448920638293193No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC736420.4284447470807613No Hit
TCCAAGACCTGAGTTACCACTGACAAGC735100.42767677898355233No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC491590.2860041188688947No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT490770.2855270477782043No Hit
TCCCGTAGATCCGAACTTGT452960.26352941614527253No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC448090.26069607930178207No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC447580.2603993643551331No Hit
TGAAAATCTTTCTTCTGAGGCGGA445450.2591601431073641No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA408770.23781993870916432No Hit
CACCCCTCCCCGCACATTGACCTCTT362980.21117959085219673No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC359810.20933530383086923No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT311110.18100193539596376No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG292490.17016893087321347No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT290360.1689297096254445No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC272990.15882394761899055No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC256000.1489392673374907No Hit
TCCAAGACCTGAGTTACCACTGACGAGC255620.14871818561253658No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACC253900.14771749991011282No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC244920.14249298967303992No Hit
GAGGTCCGGACTTGGGGAGGGTTATC242290.1409628714187524No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA242290.1409628714187524No Hit
CGGACCTCGGATCAGCCTG236900.13782700168848258No Hit
TTATTCGATTACTAAAGGACGGG232400.13520892862981576No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT228470.13292247815858005No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC224120.13039167420186878No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC206320.1200357407698089No Hit
TTAACGAGTAGATAAATGAAGAG205020.1192794085528607No Hit
ACTGGTGGTTCAGTGGTAGAATTCTC202190.11763293149596578No Hit
TGAAAACCTTTCTCCTGAGGCGGAA200510.1166555175540635No Hit
CACAACTTTCAGCGACGGATATCTCGGCT198500.11548611158785899No Hit
CGGACCTCGGATCAGCCT190250.11068631098030313No Hit
TAATACTGAATAAATCGTCGATGTAACG189140.11004051962583197No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA187120.10886529571949709No Hit
CACCCCTCCCCGCACATTGACCTCTTA185660.10801587646046297No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG179460.10440875357963311No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)