FastQCFastQC Report
Wed 18 Dec 2024
1E12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6928881
Total Bases170.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA5808478.382984207695298No Hit
CGTGGTGGACTGCTTGCAGTGCT4053515.850165416320471No Hit
CGGACTGGCCGTGGTCGAGGC3394364.898857405690761No Hit
CAGCTGTGACCCTCGGGTC2693613.887510840495024No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1512222.1824880525441266No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1263381.823353583356389No Hit
CTCGGGCTGAGACTTGAAGCG1107281.598064680285316No Hit
CGATCAAGATAGTCGAACTCGACCCTGTC978401.4120606198894163No Hit
GTTGGATCCGGCCCGGACTGGCC707171.0206121305878972No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC695661.004000501668307No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT664300.9587406682262258No Hit
CTCGGGCTGAGACTTGAAGC621360.8967681794506213No Hit
CGGACTGGCCGTGGTCGAGGT564930.8153264574756011No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA559630.8076773147063718No Hit
CAGCTGTGACCCTCGGGT508000.7331631182582007No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC498320.7191926084457216No Hit
CGTGGTGGACTGCTTGCAGTGCC494930.7143000435423844No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC448930.6479112572434135No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTC406640.586876870882903No Hit
GGACTGGCCGTGGTCGAGGC395760.5711744796887117No Hit
CTCGGGCTGAGACTTGAAGCGGTT348950.5036166734570849No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT328040.47343864038074834No Hit
CGTGGTGGACTGCTTGCAGTGCTC252870.3649507041613213No Hit
CTTTCCGAGGTCGCGGTC234070.33781789584782884No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTT212190.3062399253212748No Hit
CGAGTGTGCTCTTAACTG212170.3062110606315796No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT210750.30416166766322006No Hit
CGTGGTGGACTGCTTGCAGT199570.28802630612360064No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT188250.27168889175611477No Hit
GACCGTGGTGGACTGCTTGCAGTGCT181080.26134090050038383No Hit
GGAAGGAATCGGCCGTGGATTGGCC180720.2608213360858701No Hit
CGATCAAGATAGTCGAACTCGACCCTGTCA179640.2592626428423291No Hit
CGATCAAGATAGTCGAACTCGACCCTGTT174320.25158463538340464No Hit
CGTGGTGGACTGCTTGCAGTGCA170920.2466776381352198No Hit
CTCGGGCTGAGACTTGAAGCA166840.24078924143739805No Hit
CCGATCAAGATAGTCGAACTCGACCCTGTCA166320.24003875950532272No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC164020.23671932019037417No Hit
CTTTCCGAGGTCGCGGTCGGA159300.22990725342230586No Hit
GTTGGATCCGGCCCGGACTGGC155540.22448069175960736No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA153740.22188286968703894No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC152870.2206272556852975No Hit
GATCCGGCCCGGACTGGCC151820.2191118594762993No Hit
CGACCAAGATAGTCGAACTCGACCCTGT149640.21596560829952194No Hit
CAGCTGTGACCCTCGGGTT141390.20405892380024998No Hit
CGGACTGGCCGTGGTCGAGGCGAGG138260.19954159986295045No Hit
CCGATCAAGATAGTCGAACTCGACCCTGT132620.19140175736890272No Hit
GAGACCGAGTGTGCTCTTAACTG132360.19102651640286505No Hit
TTGGATCCGGCCCGGACTGGCC131370.1895977142629524No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT129520.18692773046614597No Hit
CCTGACTGGCGCCGACACT129180.1864370307413275No Hit
GTTGGATCCGGCCCGGACTGGCT128220.18505152563595767No Hit
TGAAAATCTTTGCTCTGAAGTGGA124520.1797115580423448No Hit
GGTTGGATCCGGCCCGGACTGGC123290.1779363796260897No Hit
GGGTCTGTAGCTCAGTCGGTTAGAGCGCA122190.17634882169285343No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA118730.171355230375583No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA117680.16983983416658477No Hit
GAGACTTGAAGCGGTTGGA116590.16826670857819612No Hit
GAGACTTGAAGCGGTTGGAT110050.15882795504786415No Hit
ACCCGTAGATCCGAACTTGTGG109730.15836612001274086No Hit
CCTGACTGGCGCCGACACTCTGTCTCGTGCA108700.15687958849343783No Hit
CGGTTAGGCGAACAAGAATAGAGGCC105840.15275193786702357No Hit
CGACCAAGATAGTCGAACTCGACCCTGTCA105790.15267977614278552No Hit
CGATCAAGATAGTCGAACTCGACCCTGT101850.1469934322728302No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG97660.1409462797816848No Hit
GTGTGTGATGACTGCATAGTTCAACTGAACA96630.13945974826238178No Hit
CTCGGGCTGAGACTTGAAGCGGTTG92450.13342702811608398No Hit
CGGACTGGCCGTGGTCGAGGCGA91450.13198379363132373No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGCGAGG88150.12722111983161494No Hit
CTCGGGCTGAGACTTGAAGCGG83160.12001937975266136No Hit
TGAAAATCTTTGCTCTGAAGTGGAT82420.11895138623393879No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT82390.11890808919939597No Hit
CCGGCCCGGACTGGCCGTGGTCGAGGC81920.11822976899155865No Hit
GACTGGCCGTGGTCGAGGC81020.11693085795527446No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCT80160.11568967629838064No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT78650.11351039222639268No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC77870.1123846693282797No Hit
CGGACTGGCCGTGGTCGAGGA76520.11043630277385338No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA76200.10997446773873011No Hit
GGACCGGTAGTTCAGTCGGTTAGAATGCC75060.10832918042610344No Hit
CTCGGGCTGAGACTTGAAGCGGT72900.10521179393902132No Hit
GAGTGTGCTCTTAACTG72850.10513963221478331No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA72310.10436028559301277No Hit
CTCGGGCTGAGACTTGAAGT71440.10310467159127137No Hit
CGGTTAGGCGAACAAGAATAGAGGC71100.10261397186645288No Hit
GGATCCGGCCCGGACTGGCC71060.10255624248706248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)