FastQCFastQC Report
Wed 18 Dec 2024
1E11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5959740
Total Bases141.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGGCTGAGACTTGAAGCG2978904.998372412219325No Hit
GTTGGATCCGGCCCGGACTGGCC2555124.287301123874531No Hit
CGGACTGGCCGTGGTCGAGGC2075643.482769382556957No Hit
CTCGGGCTGAGACTTGAAGCGGTT1752432.940447066482766No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA1675302.8110286690358977No Hit
CGTGGTGGACTGCTTGCAGTGCT1123941.8858876393936648No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAT973401.6332927275350937No Hit
CAGCTGTGACCCTCGGGT774801.3000567138834915No Hit
CAGCTGTGACCCTCGGGTC742741.2462624208438622No Hit
CTCGGGCTGAGACTTGAAGC648011.0873125337682517No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC463480.7776849325641723No Hit
GGACTGGCCGTGGTCGAGGC425640.7141922298623765No Hit
GTTGGATCCGGCCCGGACTGGCT401840.674257601841691No Hit
CTCGGGCTGAGACTTGAAGCA382390.6416219499508368No Hit
CGAGTGTGCTCTTAACTG367610.6168222103648817No Hit
TGAAAATCTTTGCTCTGAAGTGGAA353830.5937003963260142No Hit
CGGACTGGCCGTGGTCGAGGT340340.5710651806958021No Hit
CGACTGCTGCCGGTCGTTGGGGC309320.519015930225144No Hit
CCGGGAAGGAATCGGCCGTGGATTGGCC301000.5050555896733749No Hit
TGAAAATCTTTGCTCTGAGGTGGAA275710.462620852587529No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGAA270530.45392919825361505No Hit
CTTTCCGAGGTCGCGGTC262900.4411266263293365No Hit
GAGACTTGAAGCGGTTGGAT248290.4166121340863863No Hit
CTCGGGCTGAGACTTGAAGCGG243750.4089943521026085No Hit
AGACTTGAAGCGGTTGGAT240980.40434649833717584No Hit
GTTGGATCCGGCCCGGACTGGC233250.3913761338581885No Hit
GAGACTTGAAGCGGTTGGA232150.3895304157563921No Hit
CTCGGGCTGAGACTTGAAGCGGT213810.35875726122280505No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC205480.3447801414155651No Hit
CGGTTAGGCGAACAAGAATAGAGGCC194240.3259202582663002No Hit
CTCGGGCTGAGACTTGAAGCGGTTG192740.32340336994566876No Hit
CCGAGCTGTTCTTCCTCGCCGAGACC187820.31514797625399765No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC185900.3119263592035894No Hit
CGACCAAGATAGTCGAACTCGACCCTGTT184270.3091913405618366No Hit
ACGGTTAGGCGAACAAGAATAGAGGCC164910.2767067019702202No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTC160770.2697600902052774No Hit
GAGACCGAGTGTGCTCTTAACTG154330.2589542496820331No Hit
GACTGGCCGTGGTCGAGGC153730.2579474943537805No Hit
GTTTACGGTTAGGCGAACAAGAATAGAGGCC153720.25793071509830967No Hit
GAGTGTGCTCTTAACTG151410.25405470708453726No Hit
GTTGGATCCGGCCCGGACTGGCA142620.23930574152563702No Hit
GAATCGGCCGTGGATTGGC135150.22677163768889244No Hit
GGAAGGAATCGGCCGTGGATTGGCC133680.22430508713467365No Hit
CGACTGCTGCCGGTCGTTGGGGCGGT131520.22068076795296437No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT131150.22005993550054198No Hit
CGGTTAGGCGAACAAGAATAGAGGC125640.21081456573608914No Hit
CTTTCCGAGGTCGCGGTCGGA125600.21074744871420564No Hit
CGACTGCTGCCGGTCGTTGGGGCG119950.20126716937316058No Hit
GCCGAGCTGTTCTTCCTCGCCGAGACC119780.2009819220301557No Hit
GACCGTGGTGGACTGCTTGCAGTGCT118760.19927043797212632No Hit
CGTGGTGGACTGCTTGCAGTGCC116440.19537765070288304No Hit
CTCGGGCTGAGACTTGAAGCGGTC115870.19442123314104306No Hit
TTGGATCCGGCCCGGACTGGCC110910.18609872242748843No Hit
GACTTGAAGCGGTTGGAT107480.1803434378009779No Hit
CTTTCCGAGGTCGCGGTCGGACCGGGA106630.1789172010859534No Hit
AGACTTGAAGCGGTTGGATCCGGCCC105810.17754130213734157No Hit
TCTCGGGCTGAGACTTGAAGCG104650.17559490850271992No Hit
GACCAAGATAGTCGAACTCGACCCTGTC101780.17077926218257844No Hit
CCGCGACTGCTGCCGGTCGTTGGGGCGGT100740.16903421961360732No Hit
GAGTGTGCTCTTAACTGA97380.1633963897753929No Hit
GAGACCGAGTGTGCTCTTAACT96690.1622386211479024No Hit
TCTCGGGCTGAGACTTGAAGCGGTTGGA95230.15978884984915448No Hit
GAGTGTGCTCTTAACT91630.15374831787963905No Hit
GATCCGGCCCGGACTGGCC89620.15037568752999292No Hit
CGGACTGGCCGTGGTCGAGGCGAG87860.1474225385671187No Hit
GAATCGGCCGTGGATTGGCCCAGCTGTGAC86280.14477141620272024No Hit
CCGACCAAGATAGTCGAACTCGACCCTGTT85800.1439660119401182No Hit
GAGGTGTAGAATAAGTGGGAGCAGCTCGC84900.1424558789477393No Hit
CCGAGCTGTTCTTCCTCGCCGAGAC83730.14049270605764683No Hit
GACTTGAAGCGGTTGGATCCGGCCC82350.1381771688026659No Hit
AGACTTGAAGCGGTTGGA79940.13413336823418473No Hit
TCTCGGGCTGAGACTTGAAGCGGTT79670.13368032833647106No Hit
CGTGGTGGACTGCTTGCAGT77950.13079429639548035No Hit
AGTGTGCTCTTAACTG76990.12918348787027623No Hit
GGATCCGGCCCGGACTGGCC76900.12903247457103834No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT76010.12753912083413035No Hit
CTACCGATAGCCAACTCGAACGTTTCCG72870.12227043461627521No Hit
CTTTCCGAGGTCGCGGTCGGACCG71470.11992133885035253No Hit
CTCGGGCTGAGACTTGAAGT71410.11982066331752726No Hit
CGACCAAGATAGTCGAACTCGACCCTGT69680.11691785212106567No Hit
TGGGGGTAGTCGTTCTATGT69650.11686751435465306No Hit
CAGCTGTGACCCTCGGGC69600.11678361807729866No Hit
GGACTGGCCGTGGTCGAGGT69600.11678361807729866No Hit
CTGACTGGCGCCGACACT69430.11649837073429378No Hit
GACTGCTGCCGGTCGTTGGGGC69350.11636413669052677No Hit
AGTGTGCTCTTAACT68530.11498823774191491No Hit
CTGACTGGCGCCGACACTCTGTCTCGTGCA68460.11487078295361879No Hit
CTCGGGCTGAGACTTGAAGCGGTTGG67840.11383046911442446No Hit
CGTGGTGGACTGCTTGCAGTGCTC66990.11240423239939998No Hit
AGACTTGAAGCGGTTGGATCCGGCC66280.11121290526096776No Hit
GTTGGATCCGGCCCGGACT66000.11074308610778323No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGT65520.10993768184518117No Hit
GGAAGGAATCGGCCGTGGAT63650.1067999610721273No Hit
GACCAAGATAGTCGAACTCGACCCTGTT62980.1056757509555786No Hit
GGTTGGATCCGGCCCGGACTGGC62700.10520593180239407No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGAC60750.10193397698557319No Hit
CGACTGCTGCCGGTCGTTGGGGT60700.10185008070821881No Hit
GCCGAGCTGTTCTTCCTCGCCGAGAC60060.10077620835808274No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)