FastQCFastQC Report
Wed 18 Dec 2024
1E10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7499528
Total Bases198 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA157415920.990107644107738No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4248815.665436544806553No Hit
GTTGGATCCGGCCCGGACTGGCC2072632.7636805942987346No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1220051.6268357155276973No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC986621.3155761269242545No Hit
TTCTGCTGAACTACTTTGAGAACGAGT948481.2647195930197208No Hit
ACCCGTAGATCCGAACTTGTGG742080.9895022726763604No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT603390.8045706343119194No Hit
TTGTGTAACTCCCTAAGGAAGG551390.735232937326189No Hit
CTCGGGCTGAGACTTGAAGCG447910.5972509203245857No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA377150.5028983157340035No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT361940.4826170393656774No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT337340.4498149750224281No Hit
GTTGGATCCGGCCCGGACTGGCT337320.4497883066774335No Hit
TGAAAATCTTTGCTCTGAAGTGGA334130.445534705650809No Hit
ACCCGTAGATCCGAACTTGT324010.43204052308358604No Hit
TGAAAATCTTTGCTCTGAAGTGGAT294640.39287805845914564No Hit
AGGATGGACAGTGATTCAGAAA272580.3634628739301993No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC270430.36059602684328934No Hit
TACAACGGGGACTAGCTCCGATCTCTCAAG254700.3396213735051059No Hit
TCAAGTCTAGGCTGGTTAGTTT244460.325967180867916No Hit
TACAACGGGGACTAGCTCCGATCTCTCAAGG240050.32008681079662615No Hit
CACCCCTCATCCGCTACTTTGACCTCATT239880.3198601298641728No Hit
TGAAAATCTTTGCTCTGAAGTGG230070.3067793066443648No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA225670.30091227074557225No Hit
TCTCGGTGGGACCTCCATT144010.19202541813298118No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT143000.19067866671075834No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT136910.18255815565992953No Hit
GTTGGATCCGGCCCGGACTGGCA132190.1762644262412248No Hit
TGATCATGGATTCTAAACTGGTAATGGCC121830.16245022353406774No Hit
TAAGTTGAAGATTGTAGCAGAAGCAAAAGC119600.15947670306717968No Hit
CAGCTGTGACCCTCGGGTC108790.14506246259764613No Hit
TGATCTGCGCATAGGAATTGCCCTGGACTGC101100.13480848394725642No Hit
TGAAAATCTTTGCTCTGAGGTGGAA99460.13262167965770646No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA98100.1308082321980797No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC91720.12230103014483046No Hit
GTGGTCCGGACGGTCGAGGGTTATCAATCC91500.12200767834989082No Hit
CGCCCCTCCCCGCCCTTTGACCTCATT91350.12180766576243199No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA91230.12164765569246491No Hit
TAAAGAGGACTGATAATCTCAGGTTATC90280.12038090930522562No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG88960.11862079853558784No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC88580.11811409998069211No Hit
TGAAAATCTTTGCTCTGAAGTGGGA84910.11322045867419923No Hit
TCGAAGACTCTAAACGGCGGAAGCCTATT84150.11220706156440778No Hit
TGAAAATCTTTGCTCTGAAGTGGAG83150.11087364431468222No Hit
TGAAAATCCTTGCTCTGAAGTGGAA82510.11002025727485784No Hit
CGGACTGGCCGTGGTCGAGGC80930.10791345802029143No Hit
TGGACAGTGATTCAGAAA80370.10716674436044508No Hit
ACCCGTAGATCCGAACTTGTGA78300.10440657065351312No Hit
TATCTATCTTTAGACTGGACTTGGAAGT76980.10264645988387537No Hit
TATTAATGCTGTAGGTGAAAGACA76700.1022731030539522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)