FastQCFastQC Report
Fri 20 Dec 2024
1E1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1E1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10473087
Total Bases284.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC6072295.7979944213200945No Hit
TGAAAATCTTTCTTCTGAGGCGGAA5629365.375072316309413No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT3728833.560392461172145No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT2071651.978070076186706No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1315561.2561339364410895No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1221091.1659313056408298No Hit
TGAAAATCTTTCTTCTGGGGCGGAA1010950.9652836837887434No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA863090.8241027693172032No Hit
TCCAAGACCTGAGTTACCACTGACAAGC591160.5644563059583101No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC516140.4928250858605491No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA508660.4856829700736755No Hit
TAGTAACAATGACCTGATCTGGAGACTCC486420.46444758837580546No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT419520.40056957418572No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC398200.38021263453650295No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC286700.2737492775530271No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC280370.2677052143269697No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT240760.22988446481920755No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC232660.2221503554778071No Hit
GCCGATGTAGCTCAGGGGTAGAGTACTTCCT230660.2202406988503008No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT188270.1797655266303049No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA188170.1796700437989296No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT164870.15742254408848128No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA163450.15606668788295178No Hit
TCCAAGACCTGAGTTACCACTGACGAGC163070.1557038531237256No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC155770.14873360643332761No Hit
TGAAAATCTTTCTTCTGAGGCGGA153790.1468430463720964No Hit
GCGCTCGTAGTTCAACTGGATA152070.14520074167244099No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA147120.14047434151936292No Hit
TCACGTGTAACTGTGCATTTCCTTGTC145880.13929035441030901No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG143520.13703695958985157No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA133260.1272404210907443No Hit
TAGTAACAATGACCTGATCTGGAGACTCT128400.12259995548590402No Hit
GAAATTCTTGGATTTGTGGAAGGCGAA124830.11919121840580527No Hit
TGCGGGATAGAGCAGGCTGGTAGCTCGCAAG124670.11903844587560478No Hit
TCCAAGACCTGAGTTACCACTGACAAGT120930.115467387982168No Hit
TCCATCGTAGTCTAGGTGGTTAGGATCTCCG120880.11541964656648035No Hit
GCGTCGGTGGTCTAGTGGTAGAATAGCTCCC113430.10830617562901941No Hit
TTTGGTCGGTCGCCGCGGCTGGGCT112970.10786695460469296No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC112360.10728450933330355No Hit
TCCAACGTAGTCTAGGTGGTTAGGATCTCCG111260.10623419818817509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)