FastQCFastQC Report
Fri 20 Dec 2024
1D9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8967009
Total Bases246.2 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2237782.4955701505373753No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1632761.8208524157832338No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1427371.5918016810287576No Hit
TCCCGTAGATCCGAACTTGTGG935131.0428560961631688No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC870130.9703681573197931No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT552870.6165601038205716No Hit
TGAAAATCTTTCTTCTGAGGCGGAA546290.6092220940115037No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA506970.5653724670065571No Hit
GTTCGCGCTAGTCCCCGCA312010.34795325843879493No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT291710.32531471753847907No Hit
TAGTAACAATGACCTGATCTGGAGACTCC285460.3183447234189237No Hit
TCCCGTAGATCCGAACTTGT281770.31422963889073824No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC277920.30993612251309216No Hit
TGAAAATCTTTCTTCTGAGACGGAA246290.2746623762728464No Hit
TCCAAGACCTGAGTTACCACTGACAAGC228420.2547337690862137No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG228020.2542876894625622No Hit
TCCCGTAGATCCGAACTTGTGA224710.25059638057684563No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC204880.2284819832343204No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA194890.2173411446336231No Hit
TCACATCGAAAATCGTACATGGATTCACT193840.21617018562153778No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT166760.18597059510032832No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC165100.18411936466217443No Hit
TAGTAACAATGACCTGATCTGGAGACTCT164220.18313798949014104No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT159170.1775062342415403No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC156810.1748743644619962No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC146590.1634770300776993No Hit
TAATCGATCTCGAACAGGACAGACAGGATC141680.15800140269737656No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT141400.15768914696082048No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC136510.1522358235616804No Hit
TGAAAATCTTTCTTCTGGGGCGGAA127430.14210981610479034No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC126830.14144069666931303No Hit
GGCTGTTTGGTCTAGTGGTATGATTCA125440.13989056997712393No Hit
AAAGGATTCTAATAATGGTAGTTTTG124470.13880882688976892No Hit
GAGGTCCGGACTTGGGGAGGGTTATC118250.13187228874198745No Hit
GTTCGCGCTAGTCCCCGC115590.12890585924470468No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG111690.12455658291410214No Hit
TGAAAATCTTTGCTCTGAGGTGGAA110910.12368672764798162No Hit
TGAGACCATGGACATATCCAACTTCGACA108360.12084297004720303No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT107450.11982813890339576No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC104210.11621489395181826No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA103880.11584687826230575No Hit
TGTTGCTGGCAAAAGTAGAAATCTGGGAGT98930.11032664291961788No Hit
CTGGTATGATGATTGAAACTTTCGTTCTTC98670.1100366911642444No Hit
TCCAAGACCTGAGTTACCACTGACAAGT96340.10743827735647415No Hit
CACCCCTCCCCGCACATTGACCTCTT94520.10540861506885965No Hit
ATGGATGAAGAAATCTTATGTAAGCT93340.10409268017908758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)