FastQCFastQC Report
Fri 20 Dec 2024
1D8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489855
Total Bases204.9 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAA3058224.0831498073060155No Hit
TGAAAATCTTTCTTCTGAGGCGGAA3003644.0102779025762185No Hit
CACCCCTCCCCGCACATTGACCTCTT992471.3250857326343435No Hit
TGAAAATCTTTCTTCTGAGACGGAA954521.2744171949924266No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC515040.6876501614517236No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC509970.6808810050394835No Hit
TCCCGTAGATCCGAACTTGTGG494410.6601062370366315No Hit
ACCCCTCCCCGCACATTGACCTCTT406580.54284094952439No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA355790.47502922286212484No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC340650.4548152133786301No Hit
CACCCCTCCCCGCACATTGACCTCTTA306060.40863274389157067No Hit
TTAACGAGTAGATAAATGAAGAGT286060.3819299572555143No Hit
TCCAAGACCTGAGTTACCACTGACAAGC285840.38163622660251767No Hit
TAGTAACAATGACCTGATCTGGAGACTCC233330.31152806028955166No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC230390.3076027506540514No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT211700.2826489965426567No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC209160.2792577426398775No Hit
TCCCGTAGATCCGAACTTGT207130.2765474097963178No Hit
ACCCCTCCCCGCACATTGACCTCTTA201740.26935100879790064No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC195050.26041892666813976No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT194500.2596846000356482No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT185170.24722775006992792No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA183190.24458417419295836No Hit
AAAGGATTCTAATAATGGTAGTTTTG182390.2435160627275161No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT163160.2178413333769479No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT157630.21045801287207833No Hit
CACCCCTCCCCGCACATTGACCTCTA156330.20872233174073465No Hit
CCACCCCTCCCCGCACATTGACCTCTT155930.20818827600801348No Hit
CACCCCTCCCCGCACATTGACCTCT149870.20009733165728844No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGT148210.19788100036649572No Hit
AAAGGATTCTAATAATGGTAGT146640.1957848316155653No Hit
CACCCCTCCCCGAACATTGACCTCATA143750.19192627894665518No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA139350.18605166588672278No Hit
CACCCCTCCCCGAACATTGACCTCAT138590.18503695999455264No Hit
TGAAAATCTTTCTTCTGAGGCGGA126970.16952264095900388No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC126890.16941582981245965No Hit
AAAGGATTCTAATAATGGTAGTTTT124070.16565073689677573No Hit
CGGACCTCGGATCAGCCTG123800.16529024927718894No Hit
CGGACCTCGGATCAGCCT123310.16463603100460555No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA117700.15714589935319176No Hit
CACCCCTCCCCGCACATTGACCTCATA115270.15390151077691092No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG114480.15284675070478668No Hit
TGAAAATCTTTGCTCTGAGGTGGA114300.15260642562506216No Hit
CACCCCTCCCCGCACATTGACCTCAT113070.1509642042469447No Hit
TTATGGAATTTAAGGGACATACGACGAGT112630.15037674294095146No Hit
CACCCCTCCCCGCACATTGACCTCTTT110860.14801354632366048No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT109810.14661165002526752No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC105210.14047000909897456No Hit
TCCAAGACCTGAGTTACCACTGACGAGC103480.13816021805495565No Hit
AAAGGATTCTAATAATGGTAGTTTTGATACA99590.13296652605424272No Hit
AGTCTTGAAGAAAACTTGATTCTGATT94270.1258635848090517No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG93020.12419466064429818No Hit
GAATGATAGCGGTCTAGCTCAAAAAAAA89700.11976199806271283No Hit
AAAGGATTCTAATAATGGTA86740.11580998564057647No Hit
TACAAAAACAAGATGAGTGCAGG85960.1147685769617703No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT85730.11446149491545564No Hit
TTTGAACTTGATCAGAGTCTCGTCAATAGC84880.11332662648342325No Hit
TCCTGTCTGAATTCAACTCTGAACCGTTGTC84730.11312635558365283No Hit
GCCAAATGATGAAATGTGGTGAGAACTGAGA84430.11272581378411198No Hit
CACCCCTCCCCGAACATTGACCTCATT84070.11224516362466296No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAAA83800.1118846760050762No Hit
TGAAAATCTTTCTTCTGGGGCGGAA83230.1111236465859486No Hit
TGAGGTAGTGATTTGTGTAGCT81440.10873374718202156No Hit
TTCTTTATGCTGTAGGTAAAAGCCCAAAAAA77610.10362016354121675No Hit
TCTCATGGAACGTCGAAGTACATATTCAGC76760.10248529510918436No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC76480.10211145609627956No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)