FastQCFastQC Report
Fri 20 Dec 2024
1D6-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D6-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13916983
Total Bases386.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA4220013.0322735897572053No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3205672.303423091053571No Hit
TGAAAATCTTTCTTCTGAGACGGAA1073610.7714387522065667No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT1056940.7594605813630727No Hit
TCCCGTAGATCCGAACTTGTGG996050.7157082824632321No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA979550.7038522645317594No Hit
TGAAAATCTTTCTTCTGGGGCGGAA976720.7018187778198767No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC942780.677431308208108No Hit
TAGTAACAATGACCTGATCTGGAGACTCC903610.6492858401853332No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT826140.593620039630716No Hit
TCCAAGACCTGAGTTACCACTGACAAGC781950.5618674679706083No Hit
TTAACGAGTAGATAAATGAAGAGT591910.4253148832616955No Hit
TCCCGTAGATCCGAACTTGT564920.4059213121119714No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC540890.3886546387244994No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT349530.25115357258106874No Hit
TGAAAATCTTTGCTCTGAGGTGGAA347750.24987455973755232No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC320040.22996363507809128No Hit
CACCCCTCCCCGCACATTGACCTCTT317740.22831097803309813No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA299790.21541306761673848No Hit
GAGGTCCGGACTTGGGGAGGGTTATC287550.20661805795120966No Hit
TCCAAGACCTGAGTTACCACTGACGAGC269190.1934255434529165No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT265610.19085314683505758No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC248990.17891090331862874No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG242500.17424753626558287No Hit
TGAAAATCTTTCTTCTGAGGCGGA238660.17148831754698557No Hit
TTAGAACGTGAGATTTGCAACAGAATTTGC227130.16320347592578074No Hit
TTAACGAGTAGATAAATGAAGAG226990.16310287940999854No Hit
TGAGACCTAGGAATAATAATGGTAGAAGG219340.15760599836904307No Hit
TGGAAATCTTTCTTCTGAGGCGGAA212780.15289233305810607No Hit
TCACATCGAAAATCGTACATGGATTCACT210630.1513474579943081No Hit
CACCCCTCCCCGCACATTGACCTCTTA208890.1500971870124437No Hit
AGGGGTTAACCGTGAGGATACAAATGAAA208500.14981695386133617No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT200690.14420510537377246No Hit
CGGACCTCGGATCAGCCT197030.14157522503260944No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA193920.13934054528916218No Hit
TGTGTAATTCTGATCTTTATTCCAAGTGG188800.13566158699769915No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC184680.1327011752475375No Hit
TGAGACCTAGGAATAATAATGGTAGAAGGG183060.13153712985062926No Hit
TACAAAAACAAGATGAGTGCAGG171750.12341036846851075No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA167130.12009068344769838No Hit
TAATCGATCTCGAACAGGACAGACAGGATC167070.12004757065522031No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC155670.11185614008438467No Hit
CACCCCTCCCCGAACATTGACCTCATA154780.11121663366262646No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC154620.11110166621601822No Hit
TTGACTGTTTTGAAGTATCGATTGAACTGG154510.11102262609647509No Hit
TTTACGTGTTGAGATGCCTTTATCTGAAA153410.11023222490104358No Hit
CGGACCTCGGATCAGCCTG150420.10808377074255247No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC149490.10741552245914218No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT148810.10692691081105725No Hit
TAAGATGTAGGGCCTCGAAAATTGGTTCT147600.1060574694960826No Hit
TTATGGAATTTAAGGGACATACGACGAGT147200.10577005087956204No Hit
TGTGTAATTCTGATCTTTATTCTATGTGG147060.10566945436377986No Hit
TGAGACCATGGACATATCCAACTTCGACA140720.10111386929192916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)