FastQCFastQC Report
Fri 20 Dec 2024
1D4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11513059
Total Bases317.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3568913.0998798842253827No Hit
TGAAAATCTTTGCTCTGAGGTGGAA1889621.6412840410181169No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT1552881.348798785796199No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1471671.2782614941867319No Hit
TCCCGTAGATCCGAACTTGTGG1256471.0913433171844251No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC1227561.0662327014914108No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1003290.8714365139621016No Hit
AAAGGATTCTAATAATGGTAGTTTTG892780.775449860892748No Hit
TAGTAACAATGACCTGATCTGGAGACTCC820670.7128166371769658No Hit
TCCCGTAGATCCGAACTTGT621710.5400041813387737No Hit
TCCAAGACCTGAGTTACCACTGACAAGC486600.4226504875897883No Hit
ATGGATGAAGAAATCTTATGTAAGCT449510.39043489658135166No Hit
AAAGGATTCTAATAATGGTAGTTTT421960.36650554817794295No Hit
TGAAAATCTTTCTTCTGAGGCGGAA417900.36297911788691434No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT415000.36046023910760816No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC376600.3271068097540367No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA374620.3253870235529932No Hit
ATGGATGAAGAAATCTTATGTAAGCTT336490.292268110499564No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT309210.26857327839629763No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG279950.24315866009198772No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT270730.2351503627315729No Hit
TCCTGTCTGAATTCAACTCTGAACCGTTGTC265660.23074666776223418No Hit
AAAGGATTCTAATAATGGTAGTTTTGATAC255460.22188716309019174No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC219910.1910091835714557No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA215890.18751749643600368No Hit
TAGGCTGCAGCATAAACACCTTTGC214180.18603222653510243No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC212740.18478147293434352No Hit
AAAGGATTCTAATAATGGTAGT205260.1782845028415124No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT200650.174280354161305No Hit
TAGTAACAATGACCTGATCTGGAGACTCT188620.16383135012163144No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT186960.162389509165201No Hit
CACCCCTCCCCGCACATTGACCTCTT183090.15902810886316138No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT180610.15687403321740992No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT180120.1564484295615961No Hit
GTTCGCGCTAGTCCCCGCA175580.1525050814036478No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACT173370.15058552205803863No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC172830.15011648945775402No Hit
TCAGCAAATATTTCCGACTGCTTGATGAGC171920.1493260826683855No Hit
TGAAAATCTTTCTTCTGAGACGGAA169730.14742389490056465No Hit
TCCCGTAGATCCGAACTTGTGA163670.14216030683070416No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCC155640.1351856183486943No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA154180.13391749317014706No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC145270.12617845526545118No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC144120.12517958954262287No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT141810.12317317230807208No Hit
TACCACCCCTCCCCGCACATTGACCTCTT137630.11954251255031352No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT136320.11840467420517865No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC133480.11593791015923743No Hit
TTATGGAATTTAAGGGACATACGACGAGT131060.11383594924685089No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT130870.11367091925786187No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT130780.11359274715781445No Hit
TTGTAAAGATAAATCTGGAGCAGAGTTCC128880.11194244726792418No Hit
AAAGGATTCTAATAATGGTAGTT126320.10971888531101942No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC125890.10934539638857058No Hit
ATGGATGAAGAAATCTTATGTAAGCTTC123140.1069568044426768No Hit
AAAGGATTCTAATAATGGTAGTTTTGA122140.10608822555326088No Hit
AAAGGATTCTAATAATGGTA119600.1038820351741444No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTA119590.10387334938525025No Hit
TCCAAGACCTGAGTTACCACTGACGAGC117460.10202327635079435No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG116730.10138921376152071No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCT116540.1012241837725317No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)