FastQCFastQC Report
Fri 20 Dec 2024
1D3-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D3-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15206508
Total Bases421 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA3551232.3353356339272633No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2580701.6971023196121031No Hit
TGAAAATCTTTCTTCTGAGACGGAA1780451.1708473766626764No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1555541.0229435975701984No Hit
TCCCGTAGATCCGAACTTGTGG1263130.8306509291942633No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1222260.8037742787495985No Hit
TAGTAACAATGACCTGATCTGGAGACTCC1110960.7305819324199875No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC912260.5999141946329821No Hit
TGAAAATCTTTCTTCTGGGGCGGAA839560.5521057168417627No Hit
TCCAAGACCTGAGTTACCACTGACAAGC799210.5255710252478741No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT769660.5061385559393385No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA557500.36661934482262465No Hit
TCACATCGAAAATCGTACATGGATTCACT538770.3543022500629336No Hit
TCCCGTAGATCCGAACTTGT502330.3303388259816126No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT468820.30830220850178097No Hit
TAGTAACAATGACCTGATCTGGAGACTCT458150.30128547592912197No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG445250.2928022659771724No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT432140.28418095725856324No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT385220.25332574710775146No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA376040.24728885816520138No Hit
CTGGTATGATGATTGAAACTTTCGTTCTTC343010.2255678950091632No Hit
CACCCCTCCCCGCACATTGACCTCTT324350.213296833171692No Hit
TGGAAATCTTTCTTCTGAGGCGGAA295650.19442333506154078No Hit
TCCAAGACCTGAGTTACCACTGACGAGC270980.1782000180449055No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC270160.17766077524175833No Hit
TAGGCTGCAGCATAAACACCTTTGC263270.1731298204689729No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT257350.1692367504755201No Hit
TTCTCAACAATTGTCTGATTGGTTCATT254880.16761244593433286No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC252550.16608020723758538No Hit
TCCAAGACCTGAGTTACCACTGACAAGT251680.16550808377570972No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG251250.16522531011064473No Hit
TGATTCCACTTGTCAAGAACACAAATGATA245350.16134539238068332No Hit
TACCACCCCTCCCCGCACATTGACCTCTT231530.15225717830812965No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC231390.15216511246368988No Hit
TCAGTCACAAGGTATTGCTCAGCAACTCC223140.1467398037734896No Hit
TTGACATGATTTCCGGACTGTTGACCTGG223010.14665431406079554No Hit
TCCCGTAGATCCGAACTTGTGA219380.14426717823710744No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC212500.1397427995960677No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC205870.13538282424867037No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT186430.12259882413503483No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT185540.12201354840966774No Hit
GTTCGCGCTAGTCCCCGCA180430.11865314508761643No Hit
TACTAGACGTGATACACTCAAGGAAGACC175510.11541768826873336No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT165450.10880209973256187No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA163690.10764470054531915No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA161790.10639523551363667No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT159350.10479065936768651No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT152950.10058193505044025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)