FastQCFastQC Report
Fri 20 Dec 2024
1D10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1D10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8008624
Total Bases221.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA2364342.952242482603753No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1005491.255509061231992No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC573120.7156285524204907No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT385170.4809440423223765No Hit
TCCCGTAGATCCGAACTTGTGG360990.45075158978621044No Hit
TGAAAATCTTTCTTCTGGGGCGGAA345730.4316971304933282No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA335170.4185113447703376No Hit
TAGTAACAATGACCTGATCTGGAGACTCC322830.4031029550144944No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC307270.3836738995362999No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT285520.35651567610116297No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT276380.3451029789886503No Hit
TTAACGAGTAGATAAATGAAGAGT270360.3375860822033847No Hit
TCCAAGACCTGAGTTACCACTGACAAGC248630.3104528318472687No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC204950.25591162726580746No Hit
CACCCCTCCCCGCACATTGACCTCTT194410.24275081462183765No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC187580.23422250813622914No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC177200.22126148012442587No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC143520.17920681505337246No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC143230.17884470540757064No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA139030.17360035881319938No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT137640.1718647298212527No Hit
TGAGACCATGGACATATCCAACTTCGACA126500.15795472480665843No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT123170.15379670714969262No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC116770.14580532186303166No Hit
TTATTCGATTACTAAAGGACGGG110390.13783890965539147No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT108800.13585354987323664No Hit
TCCAAGACCTGAGTTACCACTGACGAGC103120.12876119543132503No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG102480.12796205690265894No Hit
CGGACCTCGGATCAGCCT100710.12575193940931675No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG97870.12220576218836095No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC97610.12188111216109034No Hit
TCAGTCACAAGGTATTGCTCAGCAACTCC97390.12160640829186138No Hit
CGAACGAGTCCTGAATAAATCCGGAGGACT93520.11677411750133355No Hit
CGGACCTCGGATCAGCCTG90150.11256615368632614No Hit
TCCCGTAGATCCGAACTTGT88530.11054333428564009No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT87800.10963181690138031No Hit
TCACATCGAAAATCGTACATGGATTCACT86330.10779629559335036No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACC86030.10742169940803814No Hit
TGCTATCGTTGTTTGACTCTCTTCGTGGT85800.10713450899929874No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC83090.10375065679197824No Hit
TACAAAAACAAGATGAGTGCAGG80990.10112848349479261No Hit
TGAAAATCTTTCTTCTGAGGCGGA80240.10019199303151202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)