FastQCFastQC Report
Fri 20 Dec 2024
1C4-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C4-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10192432
Total Bases262.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA2340082.2958995458591236No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2278912.2358844287604764No Hit
TGAAAATCTTTCTTCTGAGACGGAA703110.6898353601966636No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC519060.5092602040415869No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT485730.47655947079166194No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT434750.4265419676089083No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA390390.3830194795511022No Hit
TCCAAGACCTGAGTTACCACTGACAAGC335470.32913636313688427No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC262050.2571025246967554No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT254590.24978336867982048No Hit
CAGGCGTAGGAGCATTGAGAGGAGTCCTCCT200910.19711684120139336No Hit
TGAAAATCTTTCTTCTGGGGCGGAA172280.1690273724661592No Hit
GGGCCTGTAGTTCAATCGGTTAGAGCACC170110.1668983418285253No Hit
AGGGCTCACGGTGGATACC155950.1530056810778821No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC152310.14943440387927043No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA138200.13559079913410263No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA131420.12893880479163364No Hit
ACGGATGATGGCGTGCCTGT130760.12829126551935788No Hit
AGGGCTCACGGTGGATACCTAGGTAC120330.11805818277718214No Hit
AAGGGCTCACGGTGGATACC116080.11388842231177014No Hit
TGAAAATCTTTCTTCTGAGGCGGAG115250.11307409262087792No Hit
GCCGGTGTAGCTCAGTTGGTAGAGCACGT112580.11045450192848968No Hit
GAGGTCCGGACTTGGGGAGGGTTATC112390.11026808910768304No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT111180.1090809337751775No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC109980.10790358964376705No Hit
GCCGGTGTAGCTCAGTTGGTAGAGCAC109250.1071873719638257No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT108320.1062749302619826No Hit
CACCCCTCCCCGCACATTGACCTCTT106790.1047738164944343No Hit
ACTGCTGGGGTAGTGGGACGGC103630.1016734769483868No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)