FastQCFastQC Report
Fri 20 Dec 2024
1C10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1C10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6142187
Total Bases160.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1980313.2241121932627577No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT816521.329363628948451No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC799101.3010023954008565No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT625461.0183017872949813No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT589460.9596907420760716No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC560200.9120529869898133No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC531740.8657176995750863No Hit
GTTCGCGCTAGTCCCCGCA438990.7147128539069227No Hit
TGAAAATCTTTCTTCTGAGGCGGAA334110.5439593421691655No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG296730.48310154021686413No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT280070.4559776509572242No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT265380.43206108833873014No Hit
GCACTGTGGTTCAGTGGTAGAATTCT259220.42203208726793895No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT253820.41324043048510245No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA224270.365130530867914No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC217810.35461310442029853No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC181580.2956275997458234No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCA175870.28633123674026856No Hit
GTTCGCGCTAGTCCCCGCC167330.2724273943466716No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC161810.2634403674131055No Hit
TCCCGTAGATCCGAACTTGTGG138150.2249198860275664No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC126440.2058550154855266No Hit
GTTCGCGCTAGTCCCCGC125140.20373850551928815No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA122780.19989622588827075No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGT116330.18939508028654942No Hit
GTTCGCGCTAGTCCCCGCG112700.183485133226976No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC111520.18156399341146728No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT109400.17811245408190926No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGG106380.1731956386218785No Hit
TGAAAATCTTTCTTCTGAGACGGAA104120.1695161674498025No Hit
TCGGTACTCTGTACTTCTTCCTGAGCA100490.1636062203902291No Hit
GTTCGCGCTAGTCCCCGCT100050.16288986317088686No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC99750.1624014377940626No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG97610.1589173367727163No Hit
TACCACCCCTCCCCGCACATTGACCTCTT97490.1587219666219866No Hit
TCCAAGACCTGAGTTACCACTGACAAGC97010.1579404860190678No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCG92270.15022336506524467No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA90610.14752074464681716No Hit
GATCTATATCTGAGGTGTGCCTTAAT86160.14027576822392415No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT84670.13784992218569705No Hit
GGGAATACCCCGTGTCGTAGGCTT83920.13662885874363642No Hit
GTATTGTAAGTGCTGAGAGT82750.13472399977402186No Hit
GGCTGTTTGGTCTAGTGGTATGATTCG82440.13421929355130346No Hit
TGAAAATCTTTCTTCTGGGGCGGAA80960.1318097283589705No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT77640.12640448752211547No Hit
GAGGTCCGGACTTGGGGAGGGTTATC76800.1250368964670076No Hit
CACAACTTTCAGCGACGGATATCTCGGCT75270.12254592704520394No Hit
ATGTACAATTCTAGGCTCTTTGGTGGT73790.12013636185287098No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA71770.11684763098225436No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA71670.11668482252331296No Hit
GCTTAGGGTGCTGTCGGCT69520.11318444065607251No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCT68230.11108421153572824No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAG64810.10551616223993181No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC64700.10533707293509625No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC64010.10421369456840048No Hit
TACCTGGTTGATCCTGCC62330.10147851245818468No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)