FastQCFastQC Report
Fri 20 Dec 2024
1B9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6836681
Total Bases189 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC70331510.287374824128841No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT3676565.377697160361877No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT2573273.763917023479668No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1867322.731325331692381No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA1188471.7383727571902214No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT988481.4458477732104218No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC886831.2971645159398253No Hit
TGAAAATCTTTCTTCTGAGGCGGAA657200.9612851616157022No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC397930.5820514369472556No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC362020.5295259497993252No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC334160.4887751819925487No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA319320.4670687428592909No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC317490.4643920054190037No Hit
GTTCGCGCTAGTCCCCGCA296330.43344131457939905No Hit
GTAGAGAGGACGAAGGGTAAGTCGTCGGGCT294510.43077920411965975No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC255640.3739241307295163No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT243600.35631324614970333No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT242800.35514308770586195No Hit
TCCCGTAGATCCGAACTTGTGG184950.2705260052355814No Hit
TAGTAACAATGACCTGATCTGGAGACTCC143890.21046762310542205No Hit
GAGGTCCGGACTTGGGGAGGGT142710.208741639400756No Hit
GGCTGTTTGGTCTAGTGGTATGATTCA142130.207893274528971No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC134410.1966012455459016No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC125840.1840659232162507No Hit
TGAAAATCTTTCTTCTGGGGCGGAA122670.17942917038252917No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT117630.1720571721863284No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA114010.16676220522794613No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGT112730.1648899517177999No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC106000.1550459938089842No Hit
GTTCGCGCTAGTCCCCGC101250.14809817804867595No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGT98340.1438417267092029No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA89340.13067744421598726No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT88900.13003385707187448No Hit
GCTTAGGGTGCTGTCGGCT87640.1281908575228243No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC83960.12280812868115391No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTT83650.12235469228416536No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA83380.1219597638093689No Hit
GAGGTCCGGACTTGGGGAGGGTTATC81000.11847854243894075No Hit
GGCTTGCCGAGGTAGCTC77120.11280327398631002No Hit
GCCGCGATCGTCTAGTGGTTAGGACCCTGC76490.11188177421178491No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT73450.10743517212518765No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT72420.10592859312874185No Hit
TCCCGTAGATCCGAACTTGT71430.10448052205448814No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA70230.10272528438872604No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG69180.10118945143118421No Hit
TGTCGTTACGATCGGCTGAGATT68400.10004854694843886No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)