FastQCFastQC Report
Fri 20 Dec 2024
1B5-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B5-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12874186
Total Bases358.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCGTAGATCCGAACTTGTGG2052581.5943376924956656No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1396971.0850938459332498No Hit
TGAAAATCTTTCTTCTGAGGCGGAA976050.7581450198094077No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT902430.7009608219113814No Hit
TCCCGTAGATCCGAACTTGT736960.572432307564921No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT607900.4721851929123907No Hit
TAGTAACAATGACCTGATCTGGAGACTCC584470.45398598404590396No Hit
TGAAAATCTTTCTTCTGAGACGGAA577000.44818367545722887No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC457880.35565743729351124No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT431670.33529886860419755No Hit
TCCAAGACCTGAGTTACCACTGACAAGC383770.29809263280800824No Hit
TCACATCGAAAATCGTACATGGATTCACT368910.28655015548167473No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT360090.27969923690709453No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA356270.2767320590210519No Hit
TCCCGTAGATCCGAACTTGTGA341170.26500316214166864No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG296240.23010386831447052No Hit
TGAAAATCTTTCTTCTGGGGCGGAA261410.2030497306781182No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT223720.17377409336792243No Hit
TGAGACCATGGACATATCCAACTTCGACA211300.16412688149759525No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC197980.15378059630333135No Hit
TAATCGATCTCGAACAGGACAGACAGGATC191050.14839773170901835No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA180300.14004768922866268No Hit
TTGACATGATTTCCGGACTGTTGACCTGG162850.12649343422566678No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT160100.12435737684697115No Hit
TTAACGAGTAGATAAATGAAGAGT151560.11772394775094908No Hit
TAGTAACAATGACCTGATCTGGAGACTCT147120.11427518601952777No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT142070.11035260792410487No Hit
TACCACCCCTCCCCGCACATTGACCTCTT134250.10427843748723219No Hit
TAGAGCCTCCAGACTTGTTAAGCCAAAACT133020.10332303727785197No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG131190.10190158818584724No Hit
CACCCCTCCCCGCACATTGACCTCTT131040.10178507596519112No Hit
TAAGATGTAGGGCCTCGAAAATTGGTTCT130620.10145884174735398No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA130550.10140446937771444No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)