FastQCFastQC Report
Fri 20 Dec 2024
1B2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9380108
Total Bases256.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4859485.180622653811662No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT3420463.6465038568852295No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT2162602.3055171646211323No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1871331.9949983518313434No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1444931.5404193640414374No Hit
GCACTGTGGTTCAGTGGTAGAATTCT1365561.4558041335984617No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1246771.3291638006726576No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC1065871.136308878319951No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT976771.0413206329820508No Hit
TCCCATATGGTCTAGTGGCTAGGATCCG867830.9251812452479226No Hit
TCCAAGACCTGAGTTACCACTGACAAGC520730.5551428618945539No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC485420.5174993720754602No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA426180.4543444489125285No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT382470.4077458383208381No Hit
TGAAAATCTTTCTTCTGGGGCGGAA368510.3928632804654275No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC366050.39024070938202415No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT316840.3377786268558955No Hit
GCACTGGTGGTTCAGTGGTAGAATTCC316430.33734153167532827No Hit
TCCCGTAGATCCGAACTTGTGG311850.33245885868265057No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA294540.3140049133762639No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT277780.29613731526332104No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT264630.28211828691098223No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC262240.27957034183401724No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCA245950.2622038040500173No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA240900.25682007072839674No Hit
TGAAAATCTTTCTTCTGAGACGGAA235250.25079668592301924No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC215900.23016792557185908No Hit
GTTCGCGCTAGTCCCCGCA211270.2252319482888683No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT190500.2030893460928168No Hit
TCCCATATGGTCTAGTGGCTAGGATCCA186670.19900623745483528No Hit
TACCACCCCTCCCCGCACATTGACCTCTT184590.19678877897781133No Hit
TAGTAACAATGACCTGATCTGGAGACTCC181500.1934945738364633No Hit
TCCCGTAGATCCGAACTTGT181440.1934306086880876No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC165400.17633059235565304No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA163260.17404916873025342No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG161870.17256730945954993No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA155290.1655524648543492No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC148650.15847365510077283No Hit
TCCAAGACCTGAGTTACCACTGACGAGC147810.15757814302351317No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGT139790.14902813485729588No Hit
ATGTACAATTCTAGGCTCTTTGGTGGT136600.14562732113532167No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT136210.14521154767087968No Hit
CACAACTTTCAGCGACGGATATCTCGGCT129080.13761035587223516No Hit
GCACTGTGGTTCAGTGGTAGAATTCC127720.13616047917571952No Hit
CTCGTCGACGGACGTGCCAGACGCTCC125380.1336658383890676No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT123960.13215199654417625No Hit
GAGGTCCGGACTTGGGGAGGGTTATC121510.12954008631883557No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA114260.12181096422343965No Hit
GTTCGCGCTAGTCCCCGC112250.11966813175285401No Hit
TACCACCCCTCCCCGCACATTGACCTCTTT105670.11265328714765331No Hit
TCACATCGAAAATCGTACATGGATTCACT105300.11225883539933655No Hit
TCGGTACTCTGTACTTCTTCCTGAGCA104270.11116076701888719No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC104090.11096887157376013No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT101950.1086874479483605No Hit
TCAGTCACAAGGTATTGCTCAGCAACTCC97920.10439112214912663No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT96530.10290926287842314No Hit
TAGGAGTGAGTTGGCATTTGTA94710.1009689867110272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)