FastQCFastQC Report
Wed 18 Dec 2024
1B12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6957311
Total Bases184 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA6633829.535034440748731No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4058755.833791244922068No Hit
TGAAAATCTTTACTCTGAAGTGGAA2602393.7405112406215566No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2146463.0851862163413424No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC1330111.9118162175012732No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT708081.017749529954892No Hit
ACCCGTAGATCCGAACTTGTGG700941.0074869443093748No Hit
TTCAATGTGAAAAGTCTACTTAGGATGAGT590490.848733080927387No Hit
TTCTGCTGAACTACTTTGAGAACGAGT573420.8241977396152048No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC547540.7869994599925172No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA495140.7116830051150509No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT408210.5867353062124145No Hit
TGGAAATCTTTGCTCTGAAGTGGAA374850.5387857463896611No Hit
TTGTGTAACTCCCTAAGGAAGG372100.5348330698455193No Hit
CACCCCTCATCCGCTACTTTGACCTCATT363490.5224575989200425No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA271360.39003574800666524No Hit
ACCCGTAGATCCGAACTTGT251950.36213703828964955No Hit
CAGCTGTGACCCTCGGGTC241520.3471456141604134No Hit
CTCGGGCTGAGACTTGAAGCGGTT209710.3014239265716309No Hit
GTTGGATCCGGCCCGGACTGGCC204340.29370542728361576No Hit
GGATCCGGCCCGGACTGGCC199510.2867630899351775No Hit
TCAAGTCTAGGCTGGTTAGTTT197540.2839315361926469No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC180000.25872064652564764No Hit
CGTGGTGGACTGCTTGCAGTGCT176910.25427927542695733No Hit
CTCGGGCTGAGACTTGAAGCG155320.22324717121313106No Hit
TGAAAATCTTTGCTCTGAAGTGGA149410.21475250998553896No Hit
TGAAAATCTTTGCTCTGAAGTGGAT142780.20522296617184427No Hit
TGTGCGTGTTTTCACTGCGGCGACTGTCGC131720.18932601977976835No Hit
GTAGTCGTGGCCGAGTGG126260.1814781601684904No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA122000.1753551048673834No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG121570.17473704998957212No Hit
TAAAGAGGACTGATAATCTCAGGTTATC116540.1675072452561054No Hit
CGCCCCTCCCCGCCCTTTGACCTCATT105540.15169653907953806No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC104850.15070477660118975No Hit
TTGTCTACAAGAAGCCTGTCCATTTTGT96720.13901922739978134No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT96430.13860239969149No Hit
AGGATGGACAGTGATTCAGAAA93670.13463534977809674No Hit
ACCCCTCATCCGCTACTTTGACCTCATT93080.13378732099226268No Hit
TTACCGTGACCGATCCCGT92160.13246497102113158No Hit
TGAAAATCTTTGCTCTGAAGTGG92060.13232123732861734No Hit
TCGTCTGATTGGATACCCACGTACTTGATC86330.1240852967475509No Hit
TAGGTGCTGCCCTGCAAATTCCCATATATC86050.12368284240851099No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAA85610.12305041416144831No Hit
TTGTTTATGCCGTGAGGATTGGAGGAAAA83150.11951456532559779No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA82440.11849405610874662No Hit
CCCCCCTCGTCCACTCACATGACCACTT81720.11745917352264403No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA81340.11691298549108986No Hit
ACCCGTAGATCCGAACTTGTGA80520.11573436921247303No Hit
TTTCTGCTGAACTACTTTGAGAACGAGT79290.11396644479454779No Hit
TCCCTTTCCGAGGTCGCGGTC77500.11139361169854273No Hit
TATTAATGCTGTAGGTGAAAGACA75100.10794400307820076No Hit
CTCGGGCTGAGACTTGAAGCGGTTGGA71830.10324391133298483No Hit
TGAAAATCTTTGCTCTGAAGTGGAG71120.10222340211613366No Hit
TGAAAATCTTTACTCTGAAGTGGA70560.10141849343805387No Hit
TTGGTCTCTGAATGTAATATTTGTAACATG70360.10113102605302537No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)