FastQCFastQC Report
Wed 18 Dec 2024
1B11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4965804
Total Bases125.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAAGTGGAA62632212.612700783196438No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1006762.0273856962538193No Hit
GTTGGATCCGGCCCGGACTGGCC761471.5334274167889026No Hit
CTCGGGCTGAGACTTGAAGCG344030.6927981853492405No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC335630.675882495563659No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC155140.312416680158943No Hit
TCTCGGGCTGAGACTTGAAGCG149920.3019047872207602No Hit
CACCCCTCATCCGCTACTTTGACCTCATT147340.29670925392947445No Hit
TTCTGCTGAACTACTTTGAGAACGAGT137270.2764305639127118No Hit
GGGACCGTAGTTCAACTGGTTAGAGCACC118160.23794736965051377No Hit
TGAAAATCTTTGCTCTGAAGTGGA109200.21990396721256017No Hit
TATTAATGCTGTAGGTGAAAGACCAAAAAAA104080.2095934515337295No Hit
ACCCGTAGATCCGAACTTGTGG85870.17292265260570092No Hit
CAGCTGTGACCCTCGGGTC68440.1378225963006192No Hit
CGACCAAGATAGTCGAACTCGACCCTGTC66900.1347213865065959No Hit
TCTCGGGCTGAGACTTGAAGCGGTT62350.1255587212060726No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT58560.11792652307662567No Hit
TAGAATGTTTACTCGTGAAGGAAAAAAAAA57650.11609399001652099No Hit
TGAAAATCTTTGCTCTGAAGTGG56440.11365732517835984No Hit
CTCGGGCTGAGACTTGAAGCGGTT55160.11107969625865219No Hit
GAGGTCCGGACGGCTGAGGGTTATCTCACA51430.10356832448481655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)