FastQCFastQC Report
Fri 20 Dec 2024
1B10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6911225
Total Bases186.7 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC4067725.885671498178688No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1801942.607265716280399No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT917221.3271453324121267No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT860731.2454087372354394No Hit
TGAAAATCTTTCTTCTGAGGCGGAA682620.9876975500001809No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC508160.7352676262167706No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT416530.6026862097529743No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT388090.5615357624733676No Hit
TCCAAGACCTGAGTTACCACTGACAAGC337380.4881623735300182No Hit
TGAAAATCTTTGCTCTGAGGTGGAA327130.4733314282200333No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC319540.4623492940831763No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA311020.45002152295721815No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC283830.41067972754468274No Hit
AAAGGATTCTAATAATGGTAGTTTTG273630.39592112830938075No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC261820.3788329854692909No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT258370.37384110631617407No Hit
GAGGTCCGGACTTGGGGAGGGTTATC249420.3608911589479434No Hit
TCCCGTAGATCCGAACTTGTGG243830.3528028677984004No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT227250.3288129094335664No Hit
GCACTGTGGTTCAATGGTAGAATTCTCGCC223030.32270690073033365No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC211530.30606730355327744No Hit
TCCCGTAGATCCGAACTTGT193210.27955970178948014No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC190520.2756674829715427No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC162230.23473407391598453No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG155220.2245911542454485No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC148770.2152585106113605No Hit
TGAAAATCTTTCTTCTGAGACGGAA148740.2152151029665508No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT143640.20783580334889978No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC141870.20527475230512682No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA139590.2019757712995887No Hit
GTTCGCGCTAGTCCCCGCA138120.1998487967039128No Hit
TAGTAACAATGACCTGATCTGGAGACTCC136620.19767841446342727No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA121940.17643760693654165No Hit
TACCACCCCTCCCCGCACATTGACCTCTT121840.17629291478717593No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT117120.1694634453371146No Hit
ATTACCACCCCTCCCCGCACATTGACCTCTT114300.16538312672500172No Hit
GCACTGTGGTTCAGTGGTAGAATTCT111890.1618960459252882No Hit
GTTCGCGCTAGTCCCCGC111730.16166453848630308No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCT110650.16010186327315346No Hit
ATGGATGAAGAAATCTTATGTAAGCTTT110600.1600295171984706No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT105760.15302641716917045No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA104250.1508415657137483No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA103390.14959721322920322No Hit
TCCAAGACCTGAGTTACCACTGACGAGC102950.14896056777199412No Hit
GGGAATACCCCGTGTCGTAGGCTT101320.1466020857373331No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGC93390.13512799829263264No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA93100.1347083910594721No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC91480.13236437823974767No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT91280.13207499394101624No Hit
AAAGGATTCTAATAATGGTAGTTTT91050.1317422019974751No Hit
AAAGGATTCTAATAATGGTAGT90580.1310621488954563No Hit
CGATTCTTTGAACGCAAATGGCGCTCGT89420.12938371996281411No Hit
GAGGTCCGGACTTGGGGAGGGTTATCACT89020.1288049513653513No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT87820.12706864557296282No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT85440.12362497241805903No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA79760.11540645833408693No Hit
TTATGGAATTTAAGGGACATACGACGAGT79720.11534858147434066No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT77030.11145636265640316No Hit
ATGGATGAAGAAATCTTATGTAAGCT76670.11093547091868662No Hit
GAGGTCCGGACTTGGGGAGGGTTAT75570.10934385727566387No Hit
TAGGCTGCAGCATAAACACCTTTGC75570.10934385727566387No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC72840.1053937615979801No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC69790.10098065104232608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)