FastQCFastQC Report
Fri 20 Dec 2024
1B1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1B1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9060812
Total Bases246.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC6328006.983921529328718No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT3136963.4621179647033844No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC2061932.275656972024141No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1499411.6548296112975307No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1092121.2053224368853475No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA593030.6544998395287309No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT554740.6122409338147619No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT490400.5412318454460815No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC478400.5279879993095542No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC436790.4820649628311458No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC419790.46330284747106554No Hit
TGAAAATCTTTCTTCTGAGACGGAA380540.4199844340661742No Hit
CTGGTATGATGATTGAAACTTTCGTTCTTC363880.4015975610132955No Hit
TACCACCCCTCCCCGCACATTGACCTCTT354670.39143290910351086No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC352450.38898279756825327No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT336700.37160024951406123No Hit
TCCAAGACCTGAGTTACCACTGACAAGC336090.37092702066878774No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA319080.35215386877026034No Hit
TAGTAACAATGACCTGATCTGGAGACTCC305650.33733179763579685No Hit
CACAACTTTCAGCGACGGATATCTCGGCT297760.3286239688010302No Hit
TGAAAATCTTTCTTCTGGGGCGGAA274750.3032288938342391No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA257090.28373836693664983No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT252350.27850704771272156No Hit
TCCCGTAGATCCGAACTTGTGG237960.2626254688873359No Hit
GAGGTCCGGACTTGGGGAGGGTTATC232930.2570740900484416No Hit
TACCACCCCTCCCCGCACATTGACCTCTTT231610.2556172669734236No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC230880.2548115996667848No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC221240.24417237660377455No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA196900.21730944202351843No Hit
ATCGGTACTCTGTATTTCTTCCTGAGCA166030.183239647837302No Hit
ATTACCACCCCTCCCCGCACATTGACCTCTT157340.17364889592676683No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA156270.17246798631292648No Hit
TCCAAGACCTGAGTTACCACTGACGAGC149670.16518387093783649No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC148810.16423472863138536No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT141710.15639878633394005No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC139300.1537389805681875No Hit
TCCCGTAGATCCGAACTTGT134630.14858491711338898No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA132400.1461237690396843No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGA131170.14476627481069027No Hit
TTACCACCCCTCCCCGCACATTGACCTCTT115990.12801280944798327No Hit
TTACCACCCCTCCCCGCACATTGACCTCTTA109740.12111497291854197No Hit
GTTCGCGCTAGTCCCCGCA107420.11855449599881335No Hit
TCGGTACTCTGTATTTCTTCCTGAGCA106500.11753913446167959No Hit
TACCACCCCTCCCCGAACATTGACCTCATA106350.117373586384973No Hit
GCACTGTGGTTCAGTGGTAGAATTCT104860.1157291421563542No Hit
GCTTAGGGTGCTGTCGGCT98340.10853331908884105No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC97750.10788216332046179No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT96120.1060832075535835No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)