FastQCFastQC Report
Fri 20 Dec 2024
1A9-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A9-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13617848
Total Bases370.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTCTTCTGAGGCGGAA138158610.145406234523985No Hit
TGAAAATCTTTCTTCTGAGACGGAA4695853.448305488503029No Hit
TGAAAATCTTTCTTCTGGGGCGGAA2551211.8734311030641553No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA1515821.1131127326432195No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC1152830.8465581345892537No Hit
CACCCCTCCCCGCACATTGACCTCTT902930.6630489633898102No Hit
TTAACGAGTAGATAAATGAAGAGT847930.6226607904567594No Hit
TGAAAATCTTTCTTCTGAGGCGGA657420.482763502720841No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA610120.44802967399841737No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC575630.4227026179173097No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC535340.39311644541780755No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT507120.3723936410510677No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA474140.3481754238995765No Hit
TCCAAGACCTGAGTTACCACTGACAAGC434190.3188389237418423No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC423880.3112679771429377No Hit
TAGTAACAATGACCTGATCTGGAGACTCC393710.28911322846311693No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT383380.281527595255873No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT376250.2762918193829157No Hit
TACGATATGTCTTAATTCTCTGAATAGGC351760.2583080674714537No Hit
TCCCGTAGATCCGAACTTGTGG330590.24276229254431392No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC329930.2422776344691173No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT291890.21434370540778544No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC280460.2059503087418805No Hit
TCCCGTAGATCCGAACTTGT262540.1927911076698756No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAAA240110.17632007641736053No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA238720.17529935713777978No Hit
CGGACCTCGGATCAGCCTG228990.1681543221807146No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC227440.16701611003441952No Hit
TTCTCCAGGATAATGTGGATTCAAAGCAGC227000.16669300465095513No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGT209170.15359989331647703No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACC209160.1535925500123074No Hit
CGGACCTCGGATCAGCCT205190.15067725825695807No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT202440.14865784961030554No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC202270.14853301343942155No Hit
AGGGGTTAACCGTGAGGATACAAATGAAA196860.14456028588364328No Hit
TTATGGAATTTAAGGGACATACGACGAGT191250.14044069224447212No Hit
TACAAAAACAAGATGAGTGCAGG190330.13976510826086472No Hit
TGAAAATCTTTCTTCTGAGGTGGAA189130.13888391176050724No Hit
CACCCCTCCCCGAACATTGACCTCAT188430.13836988046863205No Hit
AGTCTTGAAGAAAACTTGATTCTGATT186380.1368645031138547No Hit
ACCCCTCCCCGCACATTGACCTCTT185670.13634312851780986No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAAAA183350.13463948195045208No Hit
TCACATCGAAAATCGTACATGGATTCACT181620.1333690903291034No Hit
CACCCCTCCCCGCACATTGACCTCTTA174230.1279423885477353No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAA173160.1271566550015832No Hit
TTAACGAGTAGATAAATGAAGAG167160.12275067249979585No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT155080.11387996106286397No Hit
CACCCCTCCCCGCACATTGACCTCT147040.10797594451046891No Hit
CCACCCCTCCCCGCACATTGACCTCTT146960.10791719807711174No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT145430.10679367253915598No Hit
TCCAAGACCTGAGTTACCACTGACGAGC144260.10593450595130743No Hit
ACAACTAGATAGAATAAGGTC143750.10555999743865552No Hit
CACCCCTCCCCGAACATTGACCTCATA142990.10500190632176244No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT142850.1048991000633874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)