FastQCFastQC Report
Fri 20 Dec 2024
1A8-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A8-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7769574
Total Bases212.6 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC79841810.27621334194127No Hit
TGAAAATCTTTGCTCTGAGGTGGAA2432333.1305834785793922No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1630672.098789457440009No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1508611.9416894671445306No Hit
TGAAAATCTTTCTTCTGAGACGGAA777751.0010201331501571No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC671530.8643073609955964No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA598430.7702224085902264No Hit
TCCAAGACCTGAGTTACCACTGACAAGC576410.7418810864019058No Hit
TGAAAATCTTTCTTCTGGGGCGGAA526720.677926486059596No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC372170.4790095312818953No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC348550.4486088941298455No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT308170.39663693273273415No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT290730.3741903996280877No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC281140.3618473805642368No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT275460.35453681244299884No Hit
TCCCGTAGATCCGAACTTGTGG265120.3412284894899No Hit
TTATGGAATTTAAGGGACATACGACGAGT256840.33057153455260224No Hit
TCCCGTAGATCCGAACTTGT231010.2973264686069017No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT211090.27168799730847537No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC201640.2595251683039508No Hit
TCCAAGACCTGAGTTACCACTGACGAGC196390.2527680410792149No Hit
TTACGTATCTGTTGTCTCAATCTGGAGC188190.24221405189010364No Hit
TAGTAACAATGACCTGATCTGGAGACTCC183260.2358687876581136No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA181370.23343622185720866No Hit
TTAACGAGTAGATAAATGAAGAGT165980.21362818604984007No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT165190.21261139928649883No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG162050.20856999367018064No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT157570.20280391177173937No Hit
CACCCCTCCCCGCACATTGACCTCTT154530.1988912133406542No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT140440.18075637094131544No Hit
TCACATCGAAAATCGTACATGGATTCACT139460.1794950405260314No Hit
GAGGTCCGGACTTGGGGAGGGTTATC129680.16690747781023774No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT129070.16612236398031605No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC115770.1490043083443185No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG109100.1404195390892731No Hit
TGCTGATGGAGGTGGCTTACTCTGATCACC105470.13574746826531287No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT103870.13368815330158385No Hit
CGGACCTCGGATCAGCCT96460.12415095087581378No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT94450.12156393645262918No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC93170.11991648448164596No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA89150.11474245563527678No Hit
GCCAAATGATGAAATGTGGTGAGAACTGAGA88670.11412466114615809No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC85330.10982584115937373No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC84930.10931101241844147No Hit
TCCAAGGCCTGAGTTACTACTGACGAGC84490.108744700803416No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC82800.1065695493729772No Hit
TGAAAATCTTTGCTCTGAGGTGGA80810.10400827638683922No Hit
TCTTTTATGTGCAGAACCTGTCGACCA79750.10264398022336875No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCA78830.10145987411922457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)