FastQCFastQC Report
Fri 20 Dec 2024
1A2-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A2-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10520184
Total Bases290 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGTTTGGTCTAGTGGTATGATTCT6390416.074427975784454No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3955543.7599532479660054No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2419262.299636584303088No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC1825571.7353023483239458No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1372981.3050912417501443No Hit
TGAAAATCTTTCTTCTGAGACGGAA996450.9471792508572094No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC878710.8352610562704986No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC847290.8053946584964673No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT634700.6033164438948977No Hit
TCCCGTAGATCCGAACTTGTGG567470.5394107175311763No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA527250.5011794470514964No Hit
TGAAAATCTTTCTTCTGGGGCGGAA475340.45183620362533583No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA412510.39211291361443873No Hit
TAGTAACAATGACCTGATCTGGAGACTCC403120.3831872142160251No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT340130.3233118356104798No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC313160.2976754018751003No Hit
TCTGAACTACCAATGTAAACTTTACAAAGT297570.2828562694340708No Hit
TACCACCCCTCCCCGCACATTGACCTCTT272910.25941561478392394No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC269250.2559365881813474No Hit
TCCCGTAGATCCGAACTTGT267550.25432064686321076No Hit
TCCAAGACCTGAGTTACCACTGACAAGC263740.2506990372031516No Hit
GGCTGTTTGGTCTAGTGGTATGATTCA257210.24449192143407378No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA246180.23400731394051666No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA240050.2281804196580592No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA233250.22171665438551263No Hit
TGTTGTGTGTTCTTCTGGTCTTGACGAGT230090.21871290464121157No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT225960.2147871177918561No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT217930.20715417144795187No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC204580.19446427933199648No Hit
GTTCGCGCTAGTCCCCGCA182800.17376121938551645No Hit
TGTCGTTACGATCGGCTGAGATTC172760.16421766007134478No Hit
CACCCCTCCCCGCACATTGACCTCTT158710.1508623803538037No Hit
TCACATCGAAAATCGTACATGGATTCACT148380.1410431604618322No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC142910.13584363163229846No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG141260.13427521799998934No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC139800.13288740957382494No Hit
TACCACCCCTCCCCGAACATTGACCTCATA136690.12993118751535143No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT134270.1276308475212981No Hit
GAGGTCCGGACTTGGGGAGGGTTATC129340.12294461769870185No Hit
TGTCGTTACGATCGGCTGAGATT115950.11021670343408442No Hit
TAGTAACAATGACCTGATCTGGAGACTCT115940.11020719789691892No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC110350.10489360262139903No Hit
GAGGTCCGGACCTGGGGAGGGTTATCCACT105760.10053056106243008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)