FastQCFastQC Report
Fri 20 Dec 2024
1A12-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A12-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6183343
Total Bases170.4 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAAATCTTTGCTCTGAGGTGGAA2425523.92266772197499No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC859141.389442571760939No Hit
AAAGGATTCTAATAATGGTAGTTTTG733011.1854590631637287No Hit
TCCAAGACCTGAGTTACCACTGACAAGC666011.077103437412416No Hit
TAGTAACAATGACCTGATCTGGAGACTCC527840.8536482611428802No Hit
TGAAAATCTTTCTTCTGAGGCGGAA444730.719238767766886No Hit
TCCCGTAGATCCGAACTTGT416610.6737617499142454No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT401560.6494221653238386No Hit
TCCCGTAGATCCGAACTTGTGG397390.6426782405569286No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC376090.6082308550568843No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC286530.46339011114214435No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT284840.4606569617761783No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT267070.43191846222989727No Hit
CACCCCTCCCCGCACATTGACCTCTT232900.37665709309672774No Hit
TTAACGAGTAGATAAATGAAGAGT225430.36457624944952916No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT207870.3361773720138119No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT184740.2987704224074259No Hit
TCCAAGACCTGAGTTACCACTGACGAGC169910.2747866324090383No Hit
TCCTGTCTGAATTCAACTCTGAACCGTTGTC162150.2622367867996325No Hit
AAAGGATTCTAATAATGGTAGTTTTGATA161450.26110471309775307No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT159260.25756293965901617No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG158730.25670579814187894No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT155130.25088370481792777No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT143190.2315737619601565No Hit
TGAAGACGAAGCTCAACCTGAGGAAAATC132810.2147867262094307No Hit
TGAAAATCTTTCTTCTGAGACGGAA132190.2137840323591947No Hit
GTGCTAACGATTTGAAGTCTCTGGATTTT130640.2112772977336046No Hit
TCATATTCTGTTCTTTCTAACTCTGAAAGC129730.2098056019211614No Hit
TTATGGAATTTAAGGGACATACGACGAGT125480.20293229730260798No Hit
TCCAAGACCTGAGTTACCACTGACAAGT102120.1651533806227473No Hit
TCTAAGGCCTGAGTTACCACTGACAAGC101790.1646196887347184No Hit
GCACTGGTGGTTCAATGGTAGAATTCTCGCC101750.16455499880889674No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT101590.16429623910561003No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT93820.15173022101474884No Hit
TGAAAATCTTTGCTCTGAGGTGGA93330.15093776942343323No Hit
AAAGGATTCTAATAATGGTAGTTTT93040.1504687674612261No Hit
TAGTAACAATGACCTGATCTGGAGACTCT92010.14880300187131784No Hit
TCTGATCTGACCTACTGACAACATTCATGT89310.14443643187835448No Hit
AAAGGATTCTAATAATGGTAGT88800.14361163532412807No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC83970.1358003267811603No Hit
TAAGATTTAGGAAAATTTGCAGGCACTCCT82380.13322890222974854No Hit
TCACGTGTAACTGTGCATTTCCTTGTC81890.13243645063843296No Hit
CACCCCTCCCCGCACATTGACCTCTTA80440.1300914408273971No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC80070.12949305901354655No Hit
GAGGTCCGGACTTGGGGAGGGTTATC76790.12418848509616884No Hit
TCCAAGGCCTGAGTTACTACTGACGAGC74910.12114805858254993No Hit
TTCGCTACCGGCTCTATACTTTGGTCGT73710.11920736080789955No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC72420.1171211107001504No Hit
TTACATCTACCTTTGAAAAATTCGAAAAGT72130.11665210873794321No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC69600.112560470929722No Hit
GCCAAATGATGAAATGTGGTGAGAACTGAGA69020.11162246700530765No Hit
TCAGCAAATATTTCCGACTGCTTGATGAGC68520.11081384293253665No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT68440.11068446308089329No Hit
ACCCCTCCCCGCACATTGACCTCTT67670.10943918200882598No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA67610.10934214712009344No Hit
TCTAAGGCCTGAGTTACCACTGACAAGCAGT67200.10867907538042124No Hit
TCGATCTGTAGTTTGAGATCATCTGCTTC65900.10657665279121666No Hit
ATGGATGAAGAAATCTTATGTAAGCTTTACC65620.1061238233104649No Hit
TTGTAAAGATAAATCTGGAGCAGAGTTCC61950.10018852261632584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)