FastQCFastQC Report
Wed 18 Dec 2024
1A11-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A11-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2615311
Total Bases74.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2406729.202423727044318No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1885997.2113412133394466No Hit
TGAAAATCTTTGCTCTGAAGTGGAA595232.2759434728795163No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC539032.0610550714618645No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT429181.641028543068109No Hit
TCAGGTCTAGGCTGGTTAGTTT407661.5587438740555137No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGC360051.376700514776254No Hit
TTGTTTAAATATTCTGTTGATGAACTCAGT201480.7703863899933889No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACT174770.6682570447644659No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA128090.48976966792859433No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC124710.476845774747248No Hit
GAGGTCCGGACGGTCGAGGGTTATCATCCTT87860.3359447499742861No Hit
TCCCGTAGATCCGAACTTGT84790.3242061842740691No Hit
TCAAGTCTAGGCTGGTTAGTTT78420.299849616355378No Hit
AAACTTGTGCTATAGGTCTGCAGTCTTGT74270.2839815226563877No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC70640.27010172021606604No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT69300.26497804658795837No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGT65510.2504864622218926No Hit
TGTGGCTATCCCAGACTGATAAATTGATATT63520.24287742452044897No Hit
TGACCACTTTGGATTCTTGCGCCATTCTT61010.2332800955603368No Hit
TCAGTGACTTTTGAGACTATTGGTAATAAT53880.20601756349436073No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCC53140.20318807208779374No Hit
CTCGGGCTGAGACTTGAAGCGGGT50090.1915259791282949No Hit
TCAGGTCTAGGCTGGTTAGTTC49450.18907885142531805No Hit
ACACTGGTGGTTCAGTGGTAGAATTCTCGCT49150.1879317603145477No Hit
TCTCAGCTCACCAATCTCTGCT45660.17458726705925223No Hit
TGGAAACGTCGTTGGTCTAAGTAGTGTT45650.17454903068889321No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT45070.1723313212080705No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT44310.1694253570607855No Hit
CTCGGGCTGAGACTTGAAGCGG43490.16628997469134646No Hit
TTCAACGTGACAACCTGTATGGCTTCCATT42170.16124277380395677No Hit
AGCTGTCGACTCCTGCACCAAGAAGAGTGTT40040.15309842691748704No Hit
GAGGTCCGGACGGAGGAGGGTTATCCAC39290.15023069914056109No Hit
TCAGGTCTAGGCTGGTTAGTT37320.1426981341798356No Hit
AGCTGTCGACTCCTGCACCTAGAAGAGCG34460.13176253225715795No Hit
TGATTAGATCTAATGGTTTTTGAGCAAATT34310.13118898670177276No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC32260.12335053077817515No Hit
TTAGACGTTAGAAATTCTTGCCTCACACA29380.11233845611477947No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA28910.11054134670790586No Hit
GAGGTCCGGACTTGGGGAGGGTTATCTACC27170.10388821826543765No Hit
GCACTTGTGGTTCAGTGGTAGAATTCTCGCC26160.10002634485917737No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)