FastQCFastQC Report
Fri 20 Dec 2024
1A10-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A10-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5951492
Total Bases162.3 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTC3870436.503293627883562No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC3539065.946508875421491No Hit
TGAAAATCTTTCTTCTGAGGCGGAA1841203.093678022250555No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1502702.524913080619112No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT1351022.270052618738293No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC1140021.915519671369801No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC970891.631338830666327No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC490630.8243815164331902No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA439290.7381174334099752No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT375850.6315223140684723No Hit
TGAAAATCTTTCTTCTGGGGCGGAA359530.6041006188028145No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGC251370.422364677630416No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA241720.4061502561038476No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC185920.31239225390876774No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTT183970.30911576458474616No Hit
TCCCGTAGATCCGAACTTGTGG175550.29496805170871443No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT170960.28725569991524813No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC163570.27483864550267395No Hit
GTTCGCGCTAGTCCCCGCA161120.2707220307109545No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT150600.25304579087059176No Hit
TCCAAGACCTGAGTTACCACTGACAAGC145900.245148611474232No Hit
TCCCATATGGTCTAGTGGTTAGGATCCGGC126580.21268616340238714No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA122300.2054946893988936No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGT121610.20433531625347057No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC115270.19368252532306185No Hit
GTTCGCGCTAGTCCCCGC113030.1899187632277755No Hit
TAGTAACAATGACCTGATCTGGAGACTCC109440.18388666236970494No Hit
TCCCGTAGATCCGAACTTGT106810.17946760240961426No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCCT106520.1789803296383495No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCG104200.1750821474682315No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA99590.16733619065605734No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT96620.16234584537793212No Hit
CACAACTTTCAGCGACGGATATCTCGGCT93410.15695223987531193No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA89560.1504832737740385No Hit
CCGGCTAGCTCAGTCGGTAGAGCATGAGACT83900.14097305347969888No Hit
TACCTGGTTGATCCTGCC83070.13957844520332044No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGT81840.13751173655278373No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGC74760.1256155599301822No Hit
ACTGGTGGTTCAGTGGTAGAATTCTC70990.11928101390374043No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG70720.11882734615118361No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA70590.11860891352958217No Hit
TGGCTTAGTTGGTCCTTACTGAAAATAGC67730.11380339585435047No Hit
AAGAGAACGAGGTATTGTACGCCGCAGAGT65140.10945154593167562No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC63920.10740163979049287No Hit
CCGGTCGAGATAGTCAAACATGACCCTGT62280.10464602825644392No Hit
TGACTGTAGTCTTTTACAATTGAACTGGGC62210.10452841069096624No Hit
CGGACCTCGGATCAGCCTGGATCACCCGCC62070.10429317556001083No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTA60920.10236088698430579No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)