FastQCFastQC Report
Fri 20 Dec 2024
1A1-fastp-adapters-polyG-31bp-merged.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1A1-fastp-adapters-polyG-31bp-merged.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8707334
Total Bases239.5 Mbp
Sequences flagged as poor quality0
Sequence length15-31
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGTGGTTCAGTGGTAGAATTCTCGCC2435512.7970788762668346No Hit
TGAAAATCTTTCTTCTGAGGCGGAA2037682.3401881678134777No Hit
GGCTGTTTGGTCTAGTGGTATGATTCT1056351.2131727116474458No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCC933591.0721881117687686No Hit
TGAAAATCTTTCTTCTGAGACGGAA847740.9735930653400915No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAAA722410.8296569305828857No Hit
TCCCGTAGATCCGAACTTGTGG680260.7812494616607104No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCT549930.6315710411476119No Hit
TCCAAGACCTGAGTTACCACTGACAAGC446120.5123497042837681No Hit
TGAAAATCTTTCTTCTGGGGCGGAA422380.4850853315147897No Hit
TCCCATATGGTCTAGTGGCTAGGATCCGGC358330.4115266509818046No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTC310500.3565959454409352No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAGT281220.3229691200544277No Hit
ATCGGTACTCTGTACTTCTTCCTGAGCA274100.3147921051380365No Hit
GCACTGGTGGTTCAGTGGTAGAATTCTCGCA271700.312035807975208No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAGT258040.2963478832901092No Hit
TCCCGTAGATCCGAACTTGT253040.29060559753421655No Hit
TACCACCCCTCCCCGCACATTGACCTCTTA231060.26536250935131234No Hit
TTGGTTGAATATAGTAACAGCAAGAACAGT222170.2551527252773352No Hit
GTTCGCGCTAGTCCCCGCA221520.25440622812906916No Hit
TCCAAGACCTGAGTTACCACTGACAAGCAG220100.2527754189743956No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCT214400.24622921321267796No Hit
TTGGTTGAATGTAATAACAGCGAGAACAGT211580.2429905640463545No Hit
TTGGTTGAATATAATAACAGCGAGAACAGT207190.2379488371526807No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACT203380.23357321540669052No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTC195520.22454634219842723No Hit
GAGGTCCGGACTTGGGGAGGGTTATCCACTC195280.22427071248214436No Hit
CACCCCTCCCCGCACATTGACCTCTT191780.2202511124530195No Hit
TTAATTGTTTACTCGCGATGGAAAAAAAAA182130.2091685009441466No Hit
TAGTAACAATGACCTGATCTGGAGACTCC177480.20382817519116642No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGA174880.20084218659810224No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCTGC171150.1965584414242063No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAGC165810.19042568023691292No Hit
TCTTTTATGTGCAGAACCTGTCGACCAAGT164420.18882932479677475No Hit
GCACTGGTGGTTCAGTGGTAGAATTCT162580.18671616363860624No Hit
TCGGTTGAATATAGTAACAGCGAGAACAGT157490.18087051673910753No Hit
TACCACCCCTCCCCGCACATTGACCTCTT156920.18021589616293573No Hit
TCTTTTATGAGTGGAACCTCTCGACCAAG156390.17960721387281114No Hit
TCAGTCACAAGGTATTGCTCAGCAACTCC150740.1731184309686524No Hit
TCGGTACTCTGTACTTCTTCCTGAGCA138180.15869380914984998No Hit
TATTAATGCTGTAGGTTAAAGGCCAAAAAA136180.15639689484749292No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAAA135900.15607532684516295No Hit
AGGGGTCAACCGTGAGGATACAAATGAAA132430.15209018053057344No Hit
TCTTTTATGTGCGGAACCTGTCTACCAAGT130550.14993108108635778No Hit
TACCACCCCTCCCCGAACATTGACCTCATA129350.14855293250494353No Hit
TCACATCGAAAATCGTACATGGATTCACT127580.1465201633473575No Hit
TTAACGAGTAGATAAATGAAGAGT124920.1434652673252226No Hit
TGATTGGTTCTCATGTGACCTCATAGGAAG118990.13665491641873392No Hit
GGGAATACCCCGTGTCGTAGGCTT118690.13631037927338036No Hit
GCACTGTGGTTCAGTGGTAGAATTCTC116840.13418573354370006No Hit
TCTTTTATGTGCGGAACCTGTCAACCAAGT114420.131406467237848No Hit
GGCTGTTTGGTCTAGTGGTATGATTCC114070.1310045072349355No Hit
TAGGCTGCAGCATAAACACCTTTGC111380.12791515749826524No Hit
TCCAAGACCTGAGTTACCACTGACGAGC110110.1264566169162685No Hit
TACGATTTGGCGATACTACATCTGAAGTAGC107100.12299976089122112No Hit
TCCAAGGCCTGAGTTACTACTGACGAGCAGT105640.12132301345050046No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAA105320.12095550716212332No Hit
GCACTGTGGTTCAGTGGTAGAATTCTCGCA104600.12012861801327479No Hit
TCCCGTAGATCCGAACTTGTGA103670.11906055286267875No Hit
GAGGTCCGGACTTGGGGAGGGTTATCC99820.1146389928306414No Hit
CGGCCTGAAAAAAAAAAAAAAAAAAAAAAAA96810.111182136805594No Hit
TATGGTCTCTTGTTATGGACAAAGCGTGT95620.10981547279569155No Hit
TCTCATGGAACTTCGAAGTACATATTCAGC95560.10974656536662084No Hit
CACCCCTCCCCGCACATTGACCTCTTA91100.10462444647236456No Hit
GGCTGTTTGGTCTAGTGGTATGATTCTCA88380.10150064302115894No Hit
GGCCGCGATCGTCTAGTGGTTAGGACCCT87460.1004440624420747No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)