FastQCFastQC Report
Fri 20 Dec 2024
2G1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename2G1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17345549
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA6775593.9062413072079756Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC3936502.2694582915767034No Hit
TTCCACCTCAGAGCAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA3814412.1990713583063872Illumina DpnII expression Sequencing Primer (100% over 25bp)
TTCCGTCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAA2521771.453842712040997Illumina DpnII expression Sequencing Primer (100% over 25bp)
GGCGAGAATTCTACCACTGAACCACAGTGCGATCGTCGGACTGTAGAACT1803921.0399901438691852No Hit
TCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTATCATT1446300.8338162141769049Illumina RNA PCR Primer (100% over 50bp)
GAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAAC1410520.813188443905696No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCGGTGGTCGCCGTA1400290.8072906772798025Illumina RNA PCR Primer (100% over 45bp)
AGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACT1217670.7020071835143413No Hit
ACTCTTCATTTATCTACTCGTTAAGATCGTCGGACTGTAGAACTCTGAAC1050340.6055386312650006Illumina DpnII expression Sequencing Primer (100% over 26bp)
GCTTTCAGAGTTAGAAAGAACAGAATATGAGATCGTCGGACTGTAGAACT998920.5758941386058175No Hit
TATCAGACTGACGTGAGATATCAGCATCAAAGCTAAGACAGGTAACTAAG888730.512367754978525No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGATCGTCGGACTG830850.4789989639417005No Hit
AGAATCATACCACTAGACCAAACAGCCGATCGTCGGACTGTAGAACTCTG689610.3975717343971067Illumina DpnII expression Sequencing Primer (100% over 23bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGGATCGTCGG683930.39429711910531057No Hit
GCTTGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT619860.3573596892205603Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTGA615770.35500173560375636Illumina DpnII expression Sequencing Primer (100% over 24bp)
CCACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGT540310.31149777963211195Illumina DpnII expression Sequencing Primer (96% over 28bp)
GGAGTCTCCAGATCAGGTCATTGTTACTAGATCGTCGGACTGTAGAACTC532920.30723732065211656Illumina DpnII expression Sequencing Primer (100% over 21bp)
TTTTTTTGGCCTTTAACCTACAGCATTAATAGATCGTCGGACTGTAGAAC505850.2916310115061795No Hit
TAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAG466910.2691814482205204No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGAGATCGTCG453130.26123704703725437No Hit
ACAAGTTCGGATCTACGGGAGATCGTCGGACTGTAGAACTCTGAACGTGT440770.2541112996769373Illumina DpnII expression Sequencing Primer (96% over 30bp)
GAACAAGATTTGTTCAGATTTTACGTTAGATCGTCGGACTGTAGAACTCT421490.24299605622168546Illumina DpnII expression Sequencing Primer (100% over 22bp)
AAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA409610.23614703691419628No Hit
AATCAAGAAAGAGCTATCAATCTGTCAATCCTTACTGATCGTCGGACTGT403610.23268793625384818No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGATCGTCGGAC402750.23219213182586496No Hit
GGCGAGAATTCTACCATTGAACCACCAGTGCGATCGTCGGACTGTAGAAC374090.2156691610049356No Hit
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGGATCGTCGGACT354380.20430601533569215No Hit
ACTTGGTAGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT353040.2035334828548811No Hit
AATCAGAATCAAGTTTTCTTCAAGACTTTGTCATTCAGAGACTCTAGTCT341100.19664987254078842No Hit
TAAGAGGTCAATGTGCGGGGAGGGGTGGATCGTCGGACTGTAGAACTCTG327080.1885671073311084Illumina DpnII expression Sequencing Primer (100% over 23bp)
TTTTTTTGGGCTTTTACCTACAGCATAAAGAAGATCGTCGGACTGTAGAA323450.18647435143159782No Hit
AACTGAATTTAAGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTC318340.18352835070253468No Hit
GATTTTCCTCAGGTTGAGCTTCGTCTTCAGATCGTCGGACTGTAGAACTC307570.1773192650172099Illumina DpnII expression Sequencing Primer (100% over 21bp)
GAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCT305190.17594715508860514Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCTCCCCGGCGGGGAATCGAACCCCGGTCTCCCGCGTGGATCGTCGGA297140.1713061950359715No Hit
GATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCTGA292630.16870610437294317Illumina DpnII expression Sequencing Primer (100% over 24bp)
AGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACTCTG282850.1630677702965758Illumina DpnII expression Sequencing Primer (100% over 23bp)
TCAAATCTCCCCGTCAAAAGGTTGAGTCTCAATAGATCGCAGTGATCGTC270320.15584401508421555No Hit
GCTACTTCAGATGTAGTATCGCCAAATCGTAGATCGTCGGACTGTAGAAC257840.14864908571069155No Hit
ACTTTTCGAATTTTTCAAAGGTAGATGTAAGATCGTCGGACTGTAGAACT229260.1321722362319002No Hit
AAAATCCAGAGACTTCAAATCGTTAGCACGATCGTCGGACTGTAGAACTC228800.13190703851460683Illumina DpnII expression Sequencing Primer (100% over 21bp)
GCTGAATATGTACTTCGAAGTTCCATGAGAGATCGTCGGACTGTAGAACT228750.1318782126757706No Hit
GCTCATCAAGCAGTCGGAAATATTTGCTGAGATCGTCGGACTGTAGAACT226030.13031008704307948No Hit
AAGAGGTCAATGTGCGGGGAGGGGTGATCGTCGGACTGTAGAACTCTGAA216300.12470057880554833Illumina DpnII expression Sequencing Primer (100% over 25bp)
GCCGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACT215300.12412406202882366No Hit
ACTTGGTCGAGAGGTTCCACTCATAAAAGAGATCGTCGGACTGTAGAACT214020.12338612055461605No Hit
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATGATCGTCGGACTGTAG211570.12197365445164059No Hit
TGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAACTCT210010.12107428827995009Illumina DpnII expression Sequencing Primer (100% over 22bp)
TAAGGGTGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGA203440.11728657305686895No Hit
GACAACGGTTCAGAGTTGAATTCAGACAGGAGATCGTCGGACTGTAGAAC200330.11549360588125519No Hit
AGAGTGGATAACCCTCCCCAAGTCCGGACCTCGATCGTCGGACTGTAGAA200050.11533218118377228No Hit
GCTCGTCAGTGGTAACTCAGGTCTTGGAGATCGTCGGACTGTAGAACTCT196370.11321059944542547Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGATCGTAGAACCAAGGAAGAATCTCAGCCGATCGTAACGACGATCGTC195080.11246689280345061No Hit
TCCGCCTCAGAAGAAAGATTTTCAGATCGTCGGACTGTAGAACTCTGAAC193930.11180389851021724Illumina DpnII expression Sequencing Primer (100% over 26bp)
TAAGAGGTCAATGTGCGGGGAGGGGTGATCGTCGGACTGTAGAACTCTGA192010.11069698629890584Illumina DpnII expression Sequencing Primer (100% over 24bp)
CGGATCCTAGCCACTAGACCATATGGGAGATCGTCGGACTGTAGAACTCT187610.10816031248131726Illumina DpnII expression Sequencing Primer (100% over 22bp)
GCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAACT187420.10805077429373958No Hit
ACTCGTCGTATGTCCCTTAAATTCCATAAGATCGTCGGACTGTAGAACTC185440.1069092710758247Illumina DpnII expression Sequencing Primer (100% over 21bp)
GGCGAGAATTCTACCATTGAACCACAGTGCGATCGTCGGACTGTAGAACT181950.10489722752505556No Hit
AGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAAC181830.1048280455118486No Hit
CATCAAGTTTTAGATCGTCGGACTGTAGAACTCTGAACGTGTAGATCTCG180550.104090104037641Illumina RNA PCR Primer (100% over 33bp)
AGGCGAGAATTCTACCACTGAACCACCAGTGCGATCGTCGGACTGTAGAA175560.10121328532178486No Hit
ACTTGGTTGACAGGTTCCGCACATAAAAGAGATCGTCGGACTGTAGAACT174790.10076936740370686No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)